LOCUS       sequence-165971-         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Drosophila P-element transgenesis vector for cloning rescue 
            transgenes. Cloning site is flanked by FRT sequences and contains 
            constitutive GFP reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Zhou Q, Neal SJ, Pignoni F
  TITLE     Mutant Analysis by Rescue Gene Excision: New tools for mosaic 
            studies in Drosophila.
  JOURNAL   Genesis. 2016 Oct 3. doi: 10.1002/dvg.22984.
  PUBMED    27696669
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genesis. 
            2016 Oct 3. doi: 10.1002/dvg.22984."
COMMENT     Sequence Label: FoRC.PGa
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(50..152)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     protein_bind    complement(297..528)
                     /gene="mutant version of attP"
                     /label=attP2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(546..565)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(547..565)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(570..586)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(570..586)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct
       61 tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat
      121 ggtgaaagtt ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc
      181 agggcttccc ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg
      241 tcacaggtat ttattcggcg caaagtgcgt cgggtgatgc tgccaactta gtcgactaca
      301 ggtcactaat accatctaag tagttgattc atagtgactg gatatgttgt gttttacagt
      361 attatgtagt ctgtttttta tgcaaagtct aatttaatat attgatattt atatcatttt
      421 acgtttctcg ttcagctttc ttgtacaaag ttggcattat aagaaagcat tgcttatcaa
      481 tttgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttgc catccagctg
      541 atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcgg ccggccaccg
      601 cggccgcagg tcgacaaagg ttaagcaatt ggcnnnccat ttaattttcc acaagtccaa
      661 ttcaaggaca aggaaaacag aataagtaca taagacccct tgaaagttaa cgacacccaa
      721 caaaagccgg tttacttaaa tgcaaataca tttgcagatt cgaagctaaa aagggaaaaa
      781 aaaaccaaca aaaactcgac agctgacttc ttttcgtgta tttgtattta ttgtattcgt
      841 ttattcacaa atgaaatgca aaattctccc gagtgttgcg cctgttcttg ttacaaaaag
      901 tataaactca aagggagcaa tgaatcctcc agttgtttag cctaa
//
