LOCUS       sequence-165974-         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone-MarkerFree backbone vector for the addition of 1 or 2 
            genes and promoters for integration into site X-2 (Chr X: 
            194944..195980).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Jessop-Fabre MM, Jakociunas T, Stovicek V, Dai Z, Jensen MK, 
            Keasling JD, Borodina I
  TITLE     EasyClone-MarkerFree: A vector toolkit for marker-less integration 
            of genes into Saccharomyces cerevisiae via CRISPR-Cas9.
  JOURNAL   Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. 
            Epub 2016 Jun 23.
  PUBMED    27166612
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/biot.201600147"; journalName: "Biotechnol J"; date: 
            "2016-08"; volume: "11"; issue: "8"; pages: "1110-7"
COMMENT     Sequence Label: pCfB2899(X-2 MarkerFree)
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    183..216
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt
       61 ggggactccc catgcgagag tagggaactg ccaggcatca aataaaacga aaggctcagt
      121 cgaaagactg ggcctttcgc ccgggctaat tatggggtgt cgcccttatt cgactctata
      181 gtgaagttcc tattctctag aaagtatagg aacttctgaa gtggggattt aaatgcggcc
      241 gcgctgaggg tttaatcgtc tatgaggaga ctgttagttg gatatcagta atgagacgaa
      301 aaagctcgaa atgaatggat atattctttt tgctactggc aactgttgaa tatttaatgt
      361 taaaacaaac taactgaggt atattcgtat ctgtatgtac acatatacta tatacaggaa
      421 aagataagca agagagagga tatcaactac gagagcgatc gattatatat caaaagctgt
      481 ccgctttgcc acccataatc ggcgcttagt ttcggagttc aatcataatt ctaccacctt
      541 acactcaact tactctttaa ctcctatagt ataatatcgc cactgacccc atattaaaaa
      601 atttttttgc tcgatcttct atcctcttta ggttaattgt cgctgttatt gtctagattt
      661 tttctcggag atggcgcatc tatttgccgt caaaagatcc tctcatacca tattaagtaa
      721 attgcctcca tttctttttc ctcgggcaga gaaactcgca ggcaacttgc tctcgaagtg
      781 gtcacgtatt aagtcctcag cgagctcgca tggaatgcgt gcgatgagcg acctcatgct
      841 atacctgaga aagcaacctg acctacagga aagagttact caagaataag aattttcgtt
      901 ttaaaaccta agagtcact
//
