LOCUS       sequence-165978-         940 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone-MarkerFree backbone vector for the addition of 1 or 2 
            genes and promoters for integration into site X-3 (Chr X: 
            223616..224744).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Jessop-Fabre MM, Jakociunas T, Stovicek V, Dai Z, Jensen MK, 
            Keasling JD, Borodina I
  TITLE     EasyClone-MarkerFree: A vector toolkit for marker-less integration 
            of genes into Saccharomyces cerevisiae via CRISPR-Cas9.
  JOURNAL   Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. 
            Epub 2016 Jun 23.
  PUBMED    27166612
REFERENCE   2  (bases 1 to 940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/biot.201600147"; journalName: "Biotechnol J"; date: 
            "2016-08"; volume: "11"; issue: "8"; pages: "1110-7"
COMMENT     Sequence Label: pCfB3034(X-3 MarkerFree)
FEATURES             Location/Qualifiers
     source          1..940
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    177..210
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 acctgacccc atgccgaact cagaagtgaa acgccgtagc gccgatggta gtgtggggac
       61 tccccatgcg agagtaggga actgccaggc atcaaataaa acgaaaggct cagtcgaaag
      121 actgggcctt tcgcccgggc taattatggg gtgtcgccct tattcgactc tatagtgaag
      181 ttcctattct ctagaaagta taggaacttc tgaagtgggg atttaaatgc ggccgcgctg
      241 agggtttaat cgagatcttt gtgttcggtt acccggctca gatcctaact tcttcttttg
      301 gtatgtttat tcgtataagt tactgttgtc cacaggcaat actctgcaga aaattaaaac
      361 ggcattaatg ctaggacaac cagaattgtt actactgtat gtgcgatagt tgataactgc
      421 aacattatgc ccggtatatt ctcaaaaaac cctattactg catacgaaga aatcgcaaga
      481 gaaatctttc ggtttggaaa agctcactgt gaggttcctt ggagccaata gtaatacagc
      541 acaatccaag gaaaaatctg gcctatatgc aaggaaggag agatagtcaa aagcattctt
      601 tcccctagaa gttggtgcat atatggcatc gttaaaacat attaccccca aaatttcttc
      661 tctaaacgat gtgcttggcc tttgttttgg tttttgatgt cggtcgtttg aggccccttg
      721 cggaaaatcg agatcgccga atggcacgcg agggaaggga aataaggttt aaaggcactg
      781 aaacaatagg caagaagtag gcgagagccg acatacgaga ctattaagtc ctcagcgagc
      841 tcgcatggaa tgcgtgcgat gagcgacctc atgctatacc tgagaaagca acctgaccta
      901 caggaaagag ttactcaaga ataagaattt tcgttttaaa
//
