LOCUS       sequence-16599-e        1331 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 1331)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD87_50
FEATURES             Location/Qualifiers
     source          1..1331
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             360..380
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(470..492)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gngnctgcgg tcgactctag aggatcccca attcttgaga cttccactat cttctctggc
       61 accctccact acgcgtaggc gtctctcgct tcgtgtattc ccgggaagcc gggttcccgt
      121 ctctcccgcc gctgccgctg ccgcacacag ctttacacct cgtagaatcc ccaaagaggg
      181 gcgtggcttg cgggtgccaa catcctcctg ccgaggaaga agcaggcact catcactcgc
      241 atcatcaacc tcgggattgg ccaaaggacc caaaggtatg tttcgaatga tactaacata
      301 acatagaaca ttttcaggag gacccttgga gggtaccgag ctcagaaaaa atgactgctc
      361 caaagaagaa gcgtaaggta ccggtgggtg aagaccagaa acagcacctc gaactgagcc
      421 gcgatattgc ccagcgtttc aacgcgctgt atggcgagat cgatcccgtc gttttacaac
      481 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
      541 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
      601 gcctgaatgg cgaatggcgc tttgcctggt ttccggcacc agaagcggtg ccggaaagct
      661 ggctggagtg cgatcttcct gaggccgata ctgtcgtcgt cccctcaaac tggcagatgc
      721 acggttacga tgcgcccatc tacaccaacg tgacctatcc cattacggtc aatccgccgt
      781 ttgttcccac ggagaatccg acgggttgtt actcgctcac atttaatgtt gatgaaagct
      841 ggctacagga aggccagacg cgaattattt ttgatggcgt taactcggcg tttcatctgt
      901 ggtgcaacgg gcgctgggtc ggttacggcc aggacagtcg tttgccgtct gaatttgacc
      961 tgagcgcatt tttacgcgcc ggagaaaacc gcctcgcggt gatggtgctg cnctggantg
     1021 acggcagtta tctggaanat caggatatgt gggggaatga ncggcatttt ccgngacgtc
     1081 tcgttgctgc ataaacngac taccnaatca ncgatttcca ngtngccnct ccctttaagg
     1141 aagattnncc ccnnctgtat ggaggctgaa ntcaaatggg ggggantggg naatacntan
     1201 ggnaannttt nttntggggg ggaaannaag tncccngggn nccccctttt gggggnaaat
     1261 nnnaaaaagg ggggntnncc atcccccccn cnnnngannn aaanccaaat ggggggcnaa
     1321 tccaattttt t
//
