LOCUS       sequence-165993-         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone-MarkerFree backbone vector for the addition of 1 or 2 
            genes and promoters for integration into site XI-3 (Chr XI: 
            93378..94567).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Jessop-Fabre MM, Jakociunas T, Stovicek V, Dai Z, Jensen MK, 
            Keasling JD, Borodina I
  TITLE     EasyClone-MarkerFree: A vector toolkit for marker-less integration 
            of genes into Saccharomyces cerevisiae via CRISPR-Cas9.
  JOURNAL   Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. 
            Epub 2016 Jun 23.
  PUBMED    27166612
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/biot.201600147"; journalName: "Biotechnol J"; date: 
            "2016-08"; volume: "11"; issue: "8"; pages: "1110-7"
COMMENT     Sequence Label: pCfB2904(XI-3 MarkerFree)
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    176..209
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 cctgacccca tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag tgtggggact
       61 ccccatgcga gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga
      121 ctgggccttt cgcccgggct aattatgggg tgtcgccctt attcgactct atagtgaagt
      181 tcctattctc tagaaagtat aggaacttct gaagtgggga tttaaatgcg gccgcgctga
      241 gggtttaata gttacttgct ctatgcgttt gcgcatcctc tttttacttt ttttttttca
      301 gtaaagccta agcataaatc gttttatacg tacgacacgt tcaacttttc ttggttagta
      361 gtggcaatct ctgcaataca tacagggagt catggtctat catcttgtcc aatcaaagaa
      421 gcatcggttc agatcgagca aactgtaggg agaaaggaaa gtagaaatgc agagtgtgct
      481 atatgtccaa tctcggtttt gtagtttgga tgtcattaga gatctaccac ccaaccggct
      541 gctttcatgt ggaacagaaa agaaatcggg gcgcttcctc ttctgtattc ctttagttaa
      601 cgtttttatt cagccatcta accatcatac ccccatacgg taacaaaacc tcttctaaga
      661 aaagaagtct ctgctcctcc gccatcttat ttttattcgc tgcgcgcgtt tattgtcgca
      721 tcgctggcca gcaaaaaatt ggttgccttt ttttacctaa aaaagacaca tctaactgat
      781 tagttttccg ttttaggata ttgacgccaa gcgtgcgtct gattattaag tcctcagcga
      841 gctcgcatgg aatgcgtgcg atgagcgacc tcatgctata cctgagaaag caacctgacc
      901 tacaggaaag agttactcaa ga
//
