LOCUS       sequence-16600-e        1376 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 1376)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD88_22
FEATURES             Location/Qualifiers
     source          1..1376
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             361..381
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(471..493)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gngcctgcng gtcgactcta gaggatcccc aattcttgag acttccacta tcttctctgg
       61 caccctccac tacgcgtagg cgtctctcgc ttcgtgtatt cccgggaagc cgggttcccg
      121 tctctcccgc cgctgccgct gccgcacaca gctttacacc tcgtagaatc cccaaagagg
      181 ggcgtggctt gcgggtgcca acatcctcct gccgaggaag aagcaggcac tcatcactcg
      241 catcatcaac ctcgggattg gccaaaggac ccaaaggtat gtttcgaatg atactaacat
      301 aacatagaac attttcagga ggacccttgg agggtaccga gctcagaaaa aatgactgct
      361 ccaaagaaga agcgtaaggt accggtgggt gaagaccaga aacagcacct cgaactgagc
      421 cgcgatattg cccagcgttt caacgcgctg tatggcgaga tcgatcccgt cgttttacaa
      481 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
      541 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
      601 agcctgaatg gcgaatggcg ctttgcctgg tttccggcac cagaagcggt gccggaaagc
      661 tggctggagt gcgatcttcc tgaggccgat actgtcgtcg tcccctcaaa ctggcagatg
      721 cacggttacg atgcgcccat ctacaccaac gtgacctatc ccattacggt caatccgccg
      781 tttgttccca cggagaatcc gacgggttgt tactcgctca catttaatgt tgatgaaagc
      841 tggctacagg aaggccagac gcgaattatt tttgatggcg ttaactcggc gtttcatctg
      901 tggtgcaacg ggcgctgggt cggttacggc caggaacagt cgtttgccgt ctgaatttga
      961 cctgagcgca tttttacgcg ccggagaaaa ccgcctcncg gtgatggtgc tgcnctggan
     1021 tgacggcagt tatctggaan atcaggatat gtggcggatg ancggcattt tccgngacgt
     1081 ctcgttgctg cataaaccga ctaccaaatc agcgattnca tgtngccctc nctttaanga
     1141 nganttcccc ccnctgtatg gagntgaant naaatggcgn cgagttgctg actacctacg
     1201 gtaaagtttt tttggggggg aaaccaggtn cccnggancg gccntttggg ggaaatnnca
     1261 aaangggggt tnccnatcnc ccaatnnntg aancnaaanc caannggggc cnaanccnan
     1321 ttttgggggg gtaanccccc cccccccctt aaaaaancgg nntttccngg gggnna
//
