LOCUS       sequence-166000-         894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone-MarkerFree backbone vector for the addition of 1 or 2 
            genes and promoters for integration into site XII-4 (Chr 
            XII:830227..831248).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Jessop-Fabre MM, Jakociunas T, Stovicek V, Dai Z, Jensen MK, 
            Keasling JD, Borodina I
  TITLE     EasyClone-MarkerFree: A vector toolkit for marker-less integration 
            of genes into Saccharomyces cerevisiae via CRISPR-Cas9.
  JOURNAL   Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. 
            Epub 2016 Jun 23.
  PUBMED    27166612
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/biot.201600147"; journalName: "Biotechnol J"; date: 
            "2016-08"; volume: "11"; issue: "8"; pages: "1110-7"
COMMENT     Sequence Label: pCfB3040(XII-4 MarkerFree)
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    181..214
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 tcccacctga ccccatgccg aactcagaag tgaaacgccg tagcgccgat ggtagtgtgg
       61 ggactcccca tgcgagagta gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg
      121 aaagactggg cctttcgccc gggctaatta tggggtgtcg cccttattcg actctatagt
      181 gaagttccta ttctctagaa agtataggaa cttctgaagt ggggatttaa atgcggccgc
      241 gctgagggtt taatgtatcc ggctgttcct tcatagccct ttcaatgaac gttgcagccc
      301 tttgaagatt ggccattttg tcaggactcg agcctgacag ttggaccaac gcaactttaa
      361 ttttttgtga aagaatcttc gaagcactca tactggcgat cttcacgccc tcctgctatt
      421 acaaaagctg tgtttttaca agaatcaaat taagttagca agatattata caacattatt
      481 gataatttca atatcgtgtt cgtacctgat gacgtatctg tgcattgata aggcccgcat
      541 ggtttcagaa agcagagcgg aacgattcca aattagtggc cttgtgcttt gcatgtcaat
      601 tgtgttacct tcagctcgtg gatttgtttt atcaatacac agtctacagt caagaatttt
      661 ttttatcaaa ttttgcgttc gagcgtataa aatagccgct gtagctactt aagttcctgt
      721 tcagcgatag tttttttcca tcacacgtac tatggcaatt aagtcctcag cgagctcgca
      781 tggaatgcgt gcgatgagcg acctcatgct atacctgaga aagcaacctg acctacagga
      841 aagagttact caagaataag aattttcgtt ttaaaaccta agagtcactt taaa
//
