LOCUS       sequence-166001-         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  yeast expression of EEF2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Davydova E, Ho AY, Malecki J, Moen A, Enserink JM, Jakobsson ME, 
            Loenarz C, Falnes PO
  TITLE     Identification and characterization of a novel evolutionarily 
            conserved lysine-specific methyltransferase targeting eukaryotic 
            translation elongation factor 2 (eEF2).
  JOURNAL   J Biol Chem. 2014 Oct 31;289(44):30499-510. doi: 
            10.1074/jbc.M114.601658. Epub 2014 Sep 17.
  PUBMED    25231979
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M114.601658"; journalName: "J Biol Chem"; date: 
            "2014-10-31- 31"; volume: "289"; issue: "44"; pages: "30499-510"
COMMENT     Sequence Label: pYES260-sc.eEF2
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..32
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        13..31
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             72..92
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 tactagcagc tgtaatacga ctcactatag ggaatattaa gcttatgcac catcaccatc
       61 accatgctag cgagaatctt tattttcagg gcgccatgga ggttgctttc actgttgacc
      121 aaatgcgttc tttaatggac aaagttacca atgtgcgtaa catgtccgtt attgctcacg
      181 tcgatcatgg taagtccact ttgaccgatt ccttggtcca aagagccggt attatttccg
      241 ctgctaaggc tggtgaagct cgtttcaccg ataccagaaa ggatgaacaa gaaagaggta
      301 tcactatcaa gtctaccgct atttctctat actctgaaat gtctgacgaa gatgtcaagg
      361 aaatcaagca aaagaccgac ggtaactcct tcttgatcaa cttgatcgac tctccaggtc
      421 acgttgactt ctcctctgaa gttactgccg ctttacgtgt cactgacggt gctttggttg
      481 tcgtcgacac cattgaaggt gtctgtgtcc aaaccgaaac tgttttgaga caagctttgg
      541 gtgagagaat caagcctgtt gttgttatca acaaggtcga cagagctttg ttggaattgc
      601 aagtttctaa ggaagattta taccaaacct ttgccagaac tgttgaatcc gttaacgtca
      661 tcgtttccac ctacgccgat gaagttttgg gtgatgtcca agtttaccca gccagaggta
      721 ccgttgcctt cggttccggt ttgcacggtt gggctttcac tatccgtcaa ttcgccacca
      781 gatatgctaa gaaattcggt gtcgacaagg ccaagatgat ggacagatta tggggtgact
      841 ctttcttcaa cccaaagacc aagaagtgga ccaacaagga cactgatgct gaaggtaagc
      901 cattggaaag agctttcaac atgttcatct tggacccaat cttcagatta ttcactgcta
      961 tcatga
//
