LOCUS       sequence-166003-        8639 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector for creating transgenic zebrafish. Expresses mCherry-Cre 
            fusion protein under the elastase promoter and Venus under the 
            crystalin promoter in zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8639)
  AUTHORS   Hesselson D, Anderson RM, Stainier DY
  TITLE     Suppression of Ptf1a activity induces acinar-to-endocrine 
            conversion.
  JOURNAL   Curr Biol. 2011 Apr 26;21(8):712-7. doi: 10.1016/j.cub.2011.03.041. 
            Epub 2011 Apr 14.
  PUBMED    21497092
REFERENCE   2  (bases 1 to 8639)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2011.03.041"; journalName: "Curr Biol"; date: 
            "2011-04-26- 26"; volume: "21"; issue: "8"; pages: "712-7"
COMMENT     Sequence Label: pHD066
FEATURES             Location/Qualifiers
     source          1..8639
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     267..286
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        267..285
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2211..2231
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2232..2939
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2377..2395)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2645..2664
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             2952..3983
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     polyA_signal    4281..4415
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4318..4337)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4372..4391
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    4433..4480
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     polyA_signal    4504..4711
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     4700..4717
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(join(4735..5448,5449..5451,5452..5454))
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4780..4801)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     5129..5148
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(6044..6064)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6044..6062)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6095..6112)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6095..6111)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6104..6126)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(6252..6707)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6389..6410)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6601..6620
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6734..6838
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6839..6907,6908..7699)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7057..7076)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7870..8458
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8359..8378
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8612..8629
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
      241 ttagggataa cagggtaatg cgcgcgtaat acgactcact atagggcgaa ttggagctca
      301 agctttttcc agtgtactta ccccactgac agactgtttt gcttgaatta tgttaattac
      361 ttgggtaaaa catgtattcc aaatgtgata atatacaata acaagctgta gttcatattt
      421 aagccatctg aaatgttaat tactgtgagc atttagtctt ttagggaaaa cagatagaaa
      481 tcacgtcatg aaaacaaata acattaggtt acaagcagta caaatttgca tggattaatt
      541 accttctagt caattttgag ttgagatatt ttccatgaga taacaaaacc cttaaactga
      601 tcagcagtgt ggtgacggaa caacagcagc acaataagaa cctcatgatc tctccggtct
      661 cctttatctt tgacagattt caggaacacc ctgttatcta gatgagatta aagcatctcc
      721 atgtgggatg atgagttttc tgcaggccgt taagggtgaa tattcataca ttatcctttg
      781 aatgaggtca tctgatacaa tagttcctgt gaggatgaat aatgagtttt aggattaaaa
      841 gccataagta aacagtcaca cttttaaatt cccagattgc aaaggctttg ctttgagcaa
      901 gccataaagt ttctgtaagg tgtctttgtt tagatcaagc aattttacat aaacaaattt
      961 caaatccaca cactaaacat ctttttagca taaaaacatt ttttaaaagg gcgacacggt
     1021 ggcacagtgg gtagcactgt cgccttacaa caagaaggtc actggtgtgt gcccaggctg
     1081 aatcagttgg catttctgtg tggagtttgc atgttctccc catgtgcgta ggtttcctcc
     1141 gggtgctctg gtttccccca cacttcaaaa acatgcgcta taggtgaatt gaataagcta
     1201 aaatagccat agtgtatgtg cgtgaatgca agagtgtatg gtgtttccct gtgctgggtt
     1261 gcagctggaa gttatgcgta aaacatatgc tggataagtt ggcggttaat tctgctgtgg
     1321 caacccctga ttaataatgg gactaagctg aaaaaaatga ataaatgaat taatattttt
     1381 acaagaatgt acacacattt tatggttcaa tggtgagata tcataaaaca caagctgggc
     1441 aatacacgtg ttttatcata ggatactatg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg
     1501 tgttaaatgt atgaaacaca taaatgtaaa tgcacatact aatatgtgct cattaagcat
     1561 tacaattttg cttaattttc agagccaaaa aaagcaacac agacgtatgt caatcctgct
     1621 gctaacctct tttctctgag aactcaccat ctgctgacct ccttcataca agaccacagg
     1681 tacattcaaa tgtaagatta ttcacagaga gaaaaataat tattcacaaa ccccatatgc
     1741 tatgggatat atagtactaa atttgggtct gtgagagctg taatattttc atttgtattc
     1801 aaaagtgaga gtgtctttag tgtattaata gattgttgaa tcaaataaag ctgatgatgt
     1861 gtgacaactg cctgaggtaa atattaaaaa taaaaaccca ttttcttcca atggttgatc
     1921 tctttcttat gggaattcta aatagtctag aactgtatgc ttatgctaat atataaatca
     1981 agagaactag tcaagtcaga ggtttgtgct tgaaggccat gtgaaaggtt tcaaagaaag
     2041 cagctgttca aaggctgata attgtttttc cagctataac aggtggaata ctcagcaata
     2101 ttgtttagaa tattgtgtgg tcaatgtgac atcacagata gagagggttt aaaaagcagt
     2161 tgatgccagt gttgcggatc taattcgata tcaagcttat cgatatggct ccaaagaaga
     2221 agcgtaaggt aatggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca
     2281 tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg
     2341 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg
     2401 gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct
     2461 acgtgaagca ccccgccgac atccccgact acaagaagct gtccttcccc gagggcttca
     2521 agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct
     2581 ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg
     2641 acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc
     2701 ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc
     2761 actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg
     2821 cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg
     2881 aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagg
     2941 cagctagcgc aatgtccaat ttactgaccg tacaccaaaa tttgcctgca ttaccggtcg
     3001 atgcaacgag tgatgaggtt cgcaagaacc tgatggacat gttcagggat cgccaggcgt
     3061 tttctgagca tacctggaaa atgcttctgt ccgtttgccg gtcgtgggcg gcatggtgca
     3121 agttgaataa ccggaaatgg tttcccgcag aacctgaaga tgttcgcgat tatcttctat
     3181 atcttcaggc gcgcggtctg gcagtaaaaa ctatccagca acatttgggc cagctaaaca
     3241 tgcttcatcg tcggtccggg ctgccacgac caagtgacag caatgctgtt tcactggtta
     3301 tgcggcggat ccgaaaagaa aacgttgatg ccggtgaacg tgcaaaacag gctctagcgt
     3361 tcgaacgcac tgatttcgac caggttcgtt cactcatgga aaatagcgat cgctgccagg
     3421 atatacgtaa tctggcattt ctggggattg cttataacac cctgttacgt atagccgaaa
     3481 ttgccaggat cagggttaaa gatatctcac gtactgacgg tgggagaatg ttaatccata
     3541 ttggcagaac gaaaacgctg gttagcaccg caggtgtaga gaaggcactt agcctggggg
     3601 taactaaact ggtcgagcga tggatttccg tctctggtgt agctgatgat ccgaataact
     3661 acctgttttg ccgggtcaga aaaaatggtg ttgccgcgcc atctgccacc agccagctat
     3721 caactcgcgc cctggaaggg atttttgaag caactcatcg attgatttac ggcgctaagg
     3781 atgactctgg tcagagatac ctggcctggt ctggacacag tgcccgtgtc ggagccgcgc
     3841 gagatatggc ccgcgctgga gtttcaatac cggagatcat gcaagctggt ggctggacca
     3901 atgtaaatat tgtcatgaac tatatccgta acctggatag tgaaacaggg gcaatggtgc
     3961 gcctgctgga agatggcgat taggcggccg ccgaaatgac cgaccaagcg acgcccaacc
     4021 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     4081 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     4141 cccacccccc ggatctaagc tctagataag taatgatcat aatcagccat atcacatctg
     4201 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     4261 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     4321 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     4381 ccaaactcat caatgtatct tatcatgtct ggatccgggg gtaccgcgtc gagaagttcc
     4441 tattccgaag ttcctattct ctagaaagta taggaacttc gggcccgcgg tggcggcgat
     4501 ctctccccag catgcctgct attgtcttcc caatcctccc ccttgctgtc ctgccccacc
     4561 ccacccccca gaatagaatg acacctactc agacaatgcg atgcaatttc ctcattttat
     4621 taggaaagga cagtgggagt ggcaccttcc agggtcaagg aaggcacggg ggaggggcaa
     4681 acaacagatg gctggcaact agaaggcaca gtcgaggctg atcagcgggc cgctttactt
     4741 gtacagctcg tccatgccga gagtgatccc ggcggcggtc acgaactcca gcaggaccat
     4801 gtgatcgcgc ttctcgttgg ggtctttgct cagggcggac tggtagctca ggtagtggtt
     4861 gtcgggcagc agcacggggc cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag
     4921 ctgcacgccg ccgtcctcga tgttgtggcg gatcttgaag ttggccttga tgccgttctt
     4981 ctgcttgtcg gcggtgatat agacgttgtg gctgttgtag ttgtactcca gcttgtgccc
     5041 caggatgttg ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt
     5101 gtcgccctcg aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat
     5161 ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg aagaagtcgt gctgcttcat
     5221 gtggtcgggg tagcgggcga agcactgcag gccgtagccc agggtggtca cgagggtggg
     5281 ccagggcacg ggcagcttgc cggtggtgca gatcagcttc agggtcagct tgccgtaggt
     5341 ggcatcgccc tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt cgccgtccag
     5401 ctcgaccagg atgggcacca ccccggtgaa cagctcctcg cccttgctca ccatgttgtc
     5461 agacctggta actccttact gtaacttatt gcacctgatg aacaaaagat gtgaactgaa
     5521 cgcaagtagg aggctaatag aggctagtac agtacaaaga tccccaaatg atgtgtgccg
     5581 gccttagtgg aggcagaccc ctggttatat acctgtgctg cagaaagagg ggcttacaaa
     5641 tggatctctc tggaatggca ttatctcatg gtgggaacag tgttgtcagc agaatcagcg
     5701 gggtggactc tgacctgaga atgacagtct agcactgaat ggctcagaca atactcttgt
     5761 ggccaactat caccggaaaa taagtgcctg ccaaaacaaa ccagaccaga tgaagctaca
     5821 tggctgtaga acgtcagtat ttaatgtgtt gtggccactt ggttcaccag atttaagagc
     5881 taattttttt aaatatcaca gataatcaat cgcttttttt ttttcttgac tgaaaagctt
     5941 agactggaaa attctttgaa aaagtttgca ctacagaaaa taaatacata acatcctgca
     6001 ctgaatgcac actattaatg ggcccggtac ccagcttttg ttccctttag tgagggttaa
     6061 ttgcgcgcta gggataacag ggtaatacgc gctcactggc cgtcgtttta caacgtcgtg
     6121 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     6181 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     6241 atggcgaatg gaaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     6301 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     6361 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     6421 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     6481 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     6541 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     6601 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     6661 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt
     6721 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     6781 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     6841 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     6901 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     6961 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     7021 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     7081 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     7141 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     7201 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     7261 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     7321 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     7381 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     7441 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     7501 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     7561 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     7621 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     7681 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     7741 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     7801 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     7861 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     7921 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     7981 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     8041 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     8101 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     8161 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     8221 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     8281 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     8341 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     8401 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     8461 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     8521 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     8581 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaag
//
