LOCUS       sequence-166007-         964 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 with sgRNA targeting Exon 1 of VEGF-A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Yiu G, Tieu E, Nguyen AT, Wong B, Smit-McBride Z
  TITLE     Genomic Disruption of VEGF-A Expression in Human Retinal Pigment 
            Epithelial Cells Using CRISPR-Cas9 Endonuclease.
  JOURNAL   Invest Ophthalmol Vis Sci. 2016 Oct 1;57(13):5490-5497. doi: 
            10.1167/iovs.16-20296.
  PUBMED    27768202
REFERENCE   2  (bases 1 to 964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1167/iovs"; 
            journalName: "Invest Ophthalmol Vis Sci"; date: "2016-10-1- 1"; 
            volume: "57"; issue: "13"; pages: "5490-5497"
COMMENT     Sequence Label: LCV2 V-2
FEATURES             Location/Qualifiers
     source          1..964
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             272..319
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             320..343
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             353..409
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     903..923
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 gagagtgatc ctggccgacg ctaatctgga caaagtgctg tccgcctaca acaagcaccg
       61 ggataagccc atcagagagc aggccgagaa tatcatccac ctgtttaccc tgaccaatct
      121 gggagcccct gccgccttca agtactttga caccaccatc gaccggaaga ggtacaccag
      181 caccaaagag gtgctggacg ccaccctgat ccaccagagc atcaccggcc tgtacgagac
      241 acggatcgac ctgtctcagc tgggaggcga caagcgacct gccgccacaa agaaggctgg
      301 acaggctaag aagaagaaag attacaaaga cgatgacgat aagggatccg gcgcaacaaa
      361 cttctctctg ctgaaacaag ccggagatgt cgaagagaat cctggaccga ccgagtacaa
      421 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
      481 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg
      541 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
      601 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
      661 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
      721 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
      781 ggccaccgtc ggagtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
      841 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
      901 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
      961 cgaa
//
