LOCUS       sequence-166008-        1020 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gene Tagging vector URA/CFP - Plasmid for yeast enhanced monomeric 
            CFP (yemCFP) labeling of endogenous proteins with a URA selection 
            marker, originating from pSIVu (ID:81089).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Wosika V, Durandau E, Varidel C, Aymoz D, Schmitt M, Pelet S
  TITLE     New families of single integration vectors and gene tagging plasmids
            for genetic manipulations in budding yeast.
  JOURNAL   Mol Genet Genomics. 2016 Dec;291(6):2231-2240. Epub 2016 Sep 16.
  PUBMED    27637489
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Genet 
            Genomics."; date: "2016-12"; volume: "291(6)"; pages: "2231-2240. 
            Epub 2016 Sep 16"
COMMENT     Sequence Label: GTU-c
FEATURES             Location/Qualifiers
     source          1..1020
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        89..107
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(136..154)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(155..188)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      complement(212..409)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
       61 ccagcaacgc ggccttcgaa ttaattaata atacgactca ctatagggga cgtccgaatt
      121 ggagctccaa ttcgccctat agtgagtcgt attaataact tcgtatagca tacattatac
      181 gaagttatcg gcgttagtat cgaatcgaca gcagtatagc gaccagcatt cacatacgat
      241 tgacgcatga tattactttc tgcgcactta acttcgcatc tgggcagatg atgtcgaggc
      301 gaaaaaaaat ataaatcacg ctaacatttg attaaaatag aacaactaca atataaaaaa
      361 actatacaaa tgacaagttc ttgaaaacaa gaatcttttt attgtcagta ctgattataa
      421 ttggccagtc tttttcaaat aagcattcca accagcattt ctataccttt taccttcaat
      481 atctggatct cttcctttac caaacaatcc tctaccaaca atgataatat cagttccagt
      541 gctaacaact tcatcaacag ttctatattg ttgtcctaat ccatcacctt tatcatctaa
      601 tccaactcca ggtgtcataa taagccaatc aaatccttct tcttggccac ccatatcacg
      661 ttgggcaata aatccaataa caaattcctt atcggattta gcaatttcaa cagttttttg
      721 agaatattct ccgtatgcta atgatcccac tgatgataat tcagctaaca tcaataaccc
      781 tcttggctct tggttggtgg tggtttcttt agctccctgt tttaatcctt caaccactcc
      841 attcccagtg acaccatgag cattggtaat atctgcccaa ctactaattt tataaactcc
      901 accaatatat tgtttcttta cggtattacc aatatcagca aattttctat cttcaaaaat
      961 cataaattga tgtttacgtg aaagttctaa taatggttca atagtggatt cataggaaaa
//
