LOCUS       sequence-166009-        1006 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gene Tagging vector TRP/GFP - Plasmid for superfolder GFP (sfGFP) 
            labeling of endogenous proteins with a TRP selection marker, 
            originating from pSIVt (ID:81092).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Wosika V, Durandau E, Varidel C, Aymoz D, Schmitt M, Pelet S
  TITLE     New families of single integration vectors and gene tagging plasmids
            for genetic manipulations in budding yeast.
  JOURNAL   Mol Genet Genomics. 2016 Dec;291(6):2231-2240. Epub 2016 Sep 16.
  PUBMED    27637489
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Genet 
            Genomics."; date: "2016-12"; volume: "291(6)"; pages: "2231-2240. 
            Epub 2016 Sep 16"
COMMENT     Sequence Label: GTT-g
FEATURES             Location/Qualifiers
     source          1..1006
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        89..107
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(136..154)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(155..188)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
       61 ccagcaacgc ggccttcgaa ttaattaata atacgactca ctatagggga cgtccgaatt
      121 ggagctccaa ttcgccctat agtgagtcgt attaataact tcgtatagca tacattatac
      181 gaagttatta tatgaagatt gttctaccat tcacaactat atgaaaggcc acagaagaaa
      241 attaccttcc atgtggatga cgatatgcgt agcatggaaa tgtatcatga ataaaatatt
      301 gttcctcagt caagcacata ccggaaagac aactgcgatt taataagagc aagaaggtga
      361 taaaacctac tcactcaaat gcctctattg taaaggaaag gacaaccctc attccaaaat
      421 ttaaatttat caatattctc atttcttagc gtttttcaca aaattagcga ttttatttga
      481 gtccttcacg ccattcgtct ccaccccacc tgatacatcg acaccgataa ccccatttaa
      541 tcggagggca tctcccacgt tttcgggggt taaacccccg gccagcataa aatgtaagga
      601 ctccggactc tcttgccgtc cgacccagtc acttatacta ttccagtcca gtagttcccc
      661 tgtaccgcct gcttccgagt cgaagagcgg tatgaatgag tgcggtttct gcgacgctgc
      721 tgacaaaagg atattacaat ctttcggaaa aaccagtcgt ttaatgacag gcaagcccag
      781 gaactcttga tattcctgcc agctctcgtc cccatgtaac tgcactatat ctatgccgta
      841 gtcgtttacg agtgccaata cgtcctcttt aggctgatta cgaaaaacac cgactaaata
      901 cttcggcgtt cctgaagagt tcttgtaggc ctttacgagc gaagaaatct tgcgcgcgat
      961 tacaggatca atggtgcgtt tcctattcgg gacgcagata atgccc
//
