LOCUS       sequence-16601-e        1365 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 1365)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD88_27
FEATURES             Location/Qualifiers
     source          1..1365
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             363..383
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(473..495)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gntgagctgc aggtcgactc tagaggatcc ccaattcttg agacttccac tatcttctct
       61 ggcaccctcc actacgcgta ggcgtctctc gcttcgtgta ttcccgggaa gccgggttcc
      121 cgtctctccc gccgctgccg ctgccgcaca cagctttaca cctcgtagaa tccccaaaga
      181 ggggcgtggc ttgcgggtgc caacatcctc ctgccgagga agaagcaggc actcatcact
      241 cgcatcatca acctcgggat tggccaaagg acccaaaggt atgtttcgaa tgatactaac
      301 ataacataga acattttcag gaggaccctt ggagggtacc gagctcagaa aaaatgactg
      361 ctccaaagaa gaagcgtaag gtaccggtgg gtgaagacca gaaacagcac ctcgaactga
      421 gccgcgatat tgcccagcgt ttcaacgcgc tgtatggcga gatcgatccc gtcgttttac
      481 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
      541 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
      601 gcagcctgaa tggcgaatgg cgctttgcct ggtttccggc accagaagcg gtgccggaaa
      661 gctggctgga gtgcgatctt cctgaggccg atactgtcgt cgtcccctca aactggcaga
      721 tgcacggtta cgatgcgccc atctacacca acgtgaccta tcccattacg gtcaatccgc
      781 cgtttgttcc cacggagaat ccgacgggtt gttactcgct cacatttaat gttgatgaaa
      841 gctggctaca ggaaggccag acgcgaatta tttttgatgg cgttaactcg gcgtttcatc
      901 tgtggtgcaa cgggcgctgg gtcggttacg gccaggtaag tttaattaag ttgatactaa
      961 ctaacaagat ctgattaatt ttcaggacag tcgtttgccg tctgaatttg acctgagcgc
     1021 atttttacgc gccgnanaaa ncgcctcncg gtgatggtgc tgcnctggaa tgacggcagt
     1081 tatctggaaa atcagganat gtgggggang ancggcattt tccgngacgt ctcgttgctg
     1141 cataancgac tacnaaatca ccatttcnng gtgcccncnc tttaagaana ttcncccncc
     1201 tgtacggagg tgaantnnaa nggnggnnng tgggggaaac nccgggaaan ttttttnggg
     1261 gggnaaanan gtnccccggn nccccctttt gggggaatna aananggggg gttnnnancn
     1321 cccnannnng aannaaacca anngggggcc anncncnnnt ttngg
//
