LOCUS       sequence-166012-         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone-MarkerFree backbone vector for the addition of 1 or 2 
            genes and promoters for integration into site XII-5 (Chr XII: 
            839226..840357).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Jessop-Fabre MM, Jakociunas T, Stovicek V, Dai Z, Jensen MK, 
            Keasling JD, Borodina I
  TITLE     EasyClone-MarkerFree: A vector toolkit for marker-less integration 
            of genes into Saccharomyces cerevisiae via CRISPR-Cas9.
  JOURNAL   Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. 
            Epub 2016 Jun 23.
  PUBMED    27166612
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/biot.201600147"; journalName: "Biotechnol J"; date: 
            "2016-08"; volume: "11"; issue: "8"; pages: "1110-7"
COMMENT     Sequence Label: pCfB2909(XII-5 MarkerFree)
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    214..247
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 attatttatg attttttgta tatacaatat ttctagtttg ttaaagagaa ttaagaaaat
       61 aaatctcgaa aataataaag ggaaaatcag tttttgatat caaaattata catgtcaacg
      121 ataatacaaa atataataca aactataaga tgttatcagt atttattatg catttagaat
      181 aaattttgtg tcgcccttat tcgactcact atagaagttc ctattctcta gaaagtatag
      241 gaacttcact tcattttatt taaatttgcg gccgcgctga ggtcttaatc tgcgatacct
      301 tttgtgatgg ctaaacaaac agacatcttt ttatatgttt ttacttctgt atatcgtgaa
      361 gtagtaagtg ataagcgaat ttggctaaga acgttgtaag tgaacaaggg acctcttttg
      421 cctttcaaaa aaggattaaa tagagttaat cattgagatt tagttttcgt tagattctgt
      481 atccctaaat aactccctta cccgacggga aggcacaaaa gacttgaata atagcaaacg
      541 gccagtagcc aagaccaaat aatactagag ttaactgatg gtcttaaaca ggcattacgt
      601 ggtgaactcc aagaccaata tacaaaatat cgataagtta ttcttgccca ccaatttaag
      661 gagcctacat caggacagta gtaccattcc tcagagaaga ggtatacata acaagaaaat
      721 cgcgtgaaca ccttatataa cttagcccgt tattgagcta aaaaaccttg caaaatttcc
      781 tatgaataag aatacttcag acgtgataaa aatttacttt ctaactcttc tcacgctgcc
      841 cctatctgtt cttccgctct accgtgagaa ataaagcatc gagtacggca gttcgctgtc
      901 actgaactaa aacaat
//
