LOCUS       sequence-16603-e        1387 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 1387)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD89_03
FEATURES             Location/Qualifiers
     source          1..1387
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             138..158
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(248..270)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gngcctgcng gtcgactcta gaggatcccc gggattggcc aaaggaccca aaggtatgtt
       61 tcgaatgata ctaacataac atagaacatt ttcaggagga cccttggagg gtaccgagct
      121 cagaaaaaat gactgctcca aagaagaagc gtaaggtacc ggtgggtgaa gaccagaaac
      181 agcacctcga actgagccgc gatattgccc agcgtttcaa cgcgctgtat ggcgagatcg
      241 atcccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
      301 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
      361 cttcccaaca gttgcgaagg taagtttaaa cagatccata ctaactaact tgttctgaca
      421 taattttcag cttgaatggc gaatggcgct ttgcctggtt tccggcacca gaagcggtgc
      481 cggaaagctg gctggagtgc gatcttcctg aggccgatac tgtcgtcgtc ccctcaaact
      541 ggcagatgca cggttacgat gcgcccatct acaccaacgt gacctatccc attacggtca
      601 atccgccgtt tgttcccacg gagaatccga cgggttgtta ctcgctcaca tttaatgttg
      661 atgaaagctg gctacaggaa ggccagacgc gaattatttt tgatggcgtt aactcggcgt
      721 ttcatctgtg gtgcaacggg cgctgggtcg gttacggcca ggtaagttta attaagttga
      781 tactaactaa caagatctga ttaattttca ggacagtcgt ttgccgtctg aatttgacct
      841 gagcgcattt ttacgcgccg gagaaaaccg cctcgcggtg atggtgctgc gctggagtga
      901 cggcagttat ctggaaaaat caggatatgt ggcggatgag cggcattttc cgtgacgtct
      961 cgttgctgca taaaccgact acacaatcag cgatttccat gttgccactc gctttatgat
     1021 gattctccng cgctgtactg gaggctgaag tccaggtaag tttaaacagg atctnactaa
     1081 ctaaatgcta acnctgaant ttnnnangng cggngagttg cgngactacc tacgggtann
     1141 ntttntttnt ggcagggnaa aacnagntcc ccnnggnncn ggccntttgg ggggnaaatn
     1201 ncaanancgg ggggntnncc catccccccc ncccttggan nccaaanccn aatngggggn
     1261 ccaancccaa tnttttnggg gggntnancc cccccccccc cccnttnaaa aaaaccnnnt
     1321 tttttcccng gggganaaan nntttttttt aaaaancntt tttnnnnnnn ccccaccccc
     1381 nggnggg
//
