LOCUS       sequence-166033-         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at 
            site XII-2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Jessop-Fabre MM, Jakociunas T, Stovicek V, Dai Z, Jensen MK, 
            Keasling JD, Borodina I
  TITLE     EasyClone-MarkerFree: A vector toolkit for marker-less integration 
            of genes into Saccharomyces cerevisiae via CRISPR-Cas9.
  JOURNAL   Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. 
            Epub 2016 Jun 23.
  PUBMED    27166612
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/biot.201600147"; journalName: "Biotechnol J"; date: 
            "2016-08"; volume: "11"; issue: "8"; pages: "1110-7"
COMMENT     Sequence Label: pCfB3048(gRNA XII-2)
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(9..31)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        155..498
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
       61 gccgggagca gacaagcccg tcagggcgcg tcagcgggct gcatttaaat gatctgttta
      121 gcttgcctcg tccccgccgg gtcacccggc cagcgacatg gaggcccaga ataccctcct
      181 tgacagtctt gacgtgcgca gctcaggggc atgatgtgac tgtcgcccgt acatttagcc
      241 catacatccc catgtataat catttgcatc catacatttt gatggccgca cggcgcgaag
      301 caaaaattac ggctcctcgc tgcagacctg cgagcaggga aacgctcccc tcacagacgc
      361 gttgaattgt ccccacgccg cgcccctgta gagaaatata aaaggttagg atttgccact
      421 gaggttcttc tttcatatac ttccttttaa aatcttgcta ggatacagtt ctcacatcac
      481 atccgaacat aaacaaccat gggtaccact cttgacgaca cggcttaccg gtaccgcacc
      541 agtgtcccgg gggacgccga ggccatcgag gcactggatg ggtccttcac caccgacacc
      601 gttttccgcg tcaccaccac cggggacggc ttcaccctgc gggaggtgcc ggtggacccg
      661 cccctgacca aggtgttccc cgacgacgaa tcggacgacg aatcggacga cggggaggac
      721 ggcgacccgg actcccggac gttcgtcgcg tacggggacg acggcgacct ggcgggcttc
      781 gtggtcgtct cgtactccgg ctggaaccgc cggctgaccg tcgaggacat cgaggtcgcc
      841 ccggagcacc gggggcacgg ggtcgggcgc gcgttgatgg
//
