LOCUS       sequence-166039-         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Example plasmid containing a bidirectional promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Jessop-Fabre MM, Jakociunas T, Stovicek V, Dai Z, Jensen MK, 
            Keasling JD, Borodina I
  TITLE     EasyClone-MarkerFree: A vector toolkit for marker-less integration 
            of genes into Saccharomyces cerevisiae via CRISPR-Cas9.
  JOURNAL   Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. 
            Epub 2016 Jun 23.
  PUBMED    27166612
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/biot.201600147"; journalName: "Biotechnol J"; date: 
            "2016-08"; volume: "11"; issue: "8"; pages: "1110-7"
COMMENT     Sequence Label: pCfB1975 (pUC19-PTEF1-PPGK1)
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    88..109
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(124..129,130..147,148..154)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    162..178
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     167..189
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     186..202
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     186..202
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        complement(274..680)
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
ORIGIN
        1 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
       61 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
      121 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
      181 tcacacagga aacagctatg accatgatta cgccaagctt gcatgcctgc aggtcgactc
      241 tagaggatcc ccacctgcac tttgtaatta aaacttagat tagattgcta tgctttcttt
      301 ctaatgagca agaagtaaaa aaagttgtaa tagaacaaga aaaatgaaac tgaaacttga
      361 gaaattgaag accgtttatt aacttaaata tcaatgggag gtcatcgaaa gagaaaaaaa
      421 tcaaaaaaaa aaattttcaa gaaaaagaaa cgtgataaaa atttttattg cctttttcga
      481 cgaagaaaaa gaaacgaggc ggtctctttt ttcttttcca aacctttagt acgggtaatt
      541 aacgacaccc tagaggaaga aagaggggaa atttagtatg ctgtgcttgg gtgttttgaa
      601 gtggtacggc gatgcgcgga gtccgagaaa atctggaaga gtaaaaaagg agtagaaaca
      661 ttttgaagct atggtgtgtg cggccggcct ggaagtacct tcaaagaatg gggtcttatc
      721 ttgttttgca agtaccactg agcaggataa taatagaaat gataatatac tatagtagag
      781 ataacgtcga tgacttccca tactgtaatt gcttttagtt gtgtattttt agtgtgcaag
      841 tttctgtaaa tcgattaatt tttttttctt tcctcttttt attaacctta atttttattt
      901 tagattcctg acttcaactc aag
//
