LOCUS       sequence-16604-e        1398 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 1398)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD89_09
FEATURES             Location/Qualifiers
     source          1..1398
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             130..150
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(240..262)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gnngtcgact ctagaggatc cccgggattg gccaaaggac ccaaaggtat gtttcgaatg
       61 atactaacat aacatagaac attttcagga ggacccttga gggtaccgag ctcagaaaaa
      121 atgactgctc caaagaagaa gcgtaaggta ccggtgggtg aagaccagaa acagcacctc
      181 gaactgagcc gcgatattgc ccagcgtttc aacgcgctgt atggcgagat cgatcccgtc
      241 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
      301 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
      361 cagttgcgaa ggtaagttta aacagatcca tactaactaa cttgttctga cataattttc
      421 agcttgaatg gcgaatggcg ctttgcctgg tttccggcac cagaagcggt gccggaaagc
      481 tggctggagt gcgatcttcc tgaggccgat actgtcgtcg tcccctcaaa ctggcagatg
      541 cacggttacg atgcgcccat ctacaccaac gtgacctatc ccattacggt caatccgccg
      601 tttgttccca cggagaatcc gacgggttgt tactcgctca catttaatgt tgatgaaagc
      661 tggctacagg aaggccagac gcgaattatt tttgatggcg ttaactcggc gtttcatctg
      721 tggtgcaacg ggcgctgggt cggttacggc caggtaagtt taattaagtt gatactaact
      781 aacaagatct gattaatttt caggacagtc gtttgccgtc tgaatttgac ctgagcgcat
      841 ttttacgcgc cggagaaaac cgcctcgcgg tgatggtgct gcgctggagt gacggcagtt
      901 atctggaaga tcaggatatg tggcggatga gcggcatttt ccgtgacgtc tcgttgctgc
      961 ataaanccga ctacacaaat cagcgatttc catgttgccn ctcgctttaa tgatgatttc
     1021 ncccngcgct gtactggagg ctgaagtcca ggtaagttta aacaggatct nactaactaa
     1081 catgctaacn ctgaattttc anatgngcgg cgaantgcgg gactacctac ggnnaaagtt
     1141 ttnttttggc agggtgaaac gcagntcncc aggggnaccg gccntttggg ggggaaatnt
     1201 ccaagaacgg gggggtntgc cnatccgctc ccntacnttg aaagtcaaaa nccgaaatgg
     1261 ggaggccnaa nccnaattnt nntggggggg gtnanccccc ccccccaccc ccttttnaaa
     1321 aaaaccnggg ntttttttcc caggggnaat naaagttnct tttnaaaaaa ccctttttnn
     1381 aggnttcccc canncncc
//
