LOCUS       sequence-166040-         993 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Example plasmid containing a bidirectional promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Jessop-Fabre MM, Jakociunas T, Stovicek V, Dai Z, Jensen MK, 
            Keasling JD, Borodina I
  TITLE     EasyClone-MarkerFree: A vector toolkit for marker-less integration 
            of genes into Saccharomyces cerevisiae via CRISPR-Cas9.
  JOURNAL   Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. 
            Epub 2016 Jun 23.
  PUBMED    27166612
REFERENCE   2  (bases 1 to 993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/biot.201600147"; journalName: "Biotechnol J"; date: 
            "2016-08"; volume: "11"; issue: "8"; pages: "1110-7"
COMMENT     Sequence Label: pCfB1975 (pUC19-PTEF1-PPGK1)
FEATURES             Location/Qualifiers
     source          1..993
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     170..192
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     184..201
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     185..201
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg
       61 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
      121 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
      181 cgttgtaaaa cgacggccag tgaattcgag ctcggtaccc atgacagatt tgttttatat
      241 ttgttgtaaa aagtagataa ttacttcctt gatgatctgt aaaaaagaga aaaagaaagc
      301 atctaagaac ttgaaaaact acgaattaga aaagaccaaa tatgtatttc ttgcattgac
      361 caatttatgc aagtttatat atatgtaaat gtaagtttca cgaggttcta ctaaactaaa
      421 ccaccccctt ggttagaaga aaagagtgtg tgagaacagg ctgttgttgt cacacgattc
      481 ggacaattct gtttgaaaga gagagagtaa cagtacgatc gaacgaactt tgctctggag
      541 atcacagtgg gcatcatagc atgtggtact aaaccctttc ccgccattcc agaaccttcg
      601 attgcttgtt acaaaacctg tgagccgtcg ctaggacctt gttgtgtgac gaaattggaa
      661 gctgcaatca ataggaagac aggaagtcga gcgtgtctgg gttttttcag ttttgttctt
      721 tttgcaaaca aatcacgagc gacggtaatt tctttctcga taagaggcca cgtgctttat
      781 gagggtaaca tcaattcaag aaggagggaa acacttcctt tttctggccc tgataatagt
      841 atgagggtga agccaaaata aaggattcgc gcccaaatcg gcatctttaa atgcaggtat
      901 gcgatagttc ctcactcttt ccttactcac gagtaattct tgcaaatgcc tattatgcag
      961 atgttataat atctgtgcgt cttgagttga gtc
//
