LOCUS       sequence-166045-         971 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cas9 protein, under the control of the TEF1 promoter, and 
            CYC terminator. KanMX resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Jessop-Fabre MM, Jakociunas T, Stovicek V, Dai Z, Jensen MK, 
            Keasling JD, Borodina I
  TITLE     EasyClone-MarkerFree: A vector toolkit for marker-less integration 
            of genes into Saccharomyces cerevisiae via CRISPR-Cas9.
  JOURNAL   Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. 
            Epub 2016 Jun 23.
  PUBMED    27166612
REFERENCE   2  (bases 1 to 971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/biot.201600147"; journalName: "Biotechnol J"; date: 
            "2016-08"; volume: "11"; issue: "8"; pages: "1110-7"
COMMENT     Sequence Label: pCfB2312 (TEF1p-Cas9-CYC1t_kanMX)
FEATURES             Location/Qualifiers
     source          1..971
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(3..23)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cacaccttcc tcttcttctt ggggtcagcc ctgctgtctc caccgagctg agagaggtcg
       61 attcttgttt catagagccc cgtaattgac tgatgaatca gtgtggcgtc caggacctcc
      121 tttgtagagg tgtaccgctt tctgtctatg gtggtgtcga agtacttgaa ggctgcaggc
      181 gcgcccaagt tggtcagagt aaacaagtgg ataatgtttt ctgcctgctc cctgatgggc
      241 ttatccctgt gcttattgta agcagaaagc accttatcga ggttagcgtc ggcgaggatc
      301 actcttttgg agaattcgct tatttgctcg atgatctcat caaggtagtg tttgtgttgt
      361 tccacgaaca gctgcttctg ctcattatct tcgggagacc ctttgagctt ttcatagtgg
      421 ctggccagat acaagaaatt aacgtattta gagggcagtg ccagctcgtt acctttctgc
      481 agctcgcccg cactagcgag cattcgtttc cggccgtttt caagctcaaa gagagagtac
      541 ttgggaagct taatgatgag gtcttttttg acctctttat atcctttcgc ctcgagaaag
      601 tcgatggggt ttttttcgaa gcttgatcgc tccatgattg tgatgcccag cagttccttg
      661 acgcttttga gttttttaga cttccctttc tccactttgg ccacaaccag tacactgtaa
      721 gcgactgtag gagaatcgaa tccgccgtat ttcttggggt cccaatcttt tttgcgtgcg
      781 atcagcttgt cgctgttcct tttcgggagg atactttcct tggagaagcc tccggtctgt
      841 acttcggtct ttttaacgat gttcacctgc ggcatggaca ggaccttccg gactgtcgcg
      901 aaatccctac ccttgtccca cacgatttct cctgtttctc cgtttgtttc gataagtggt
      961 cgcttccgaa t
//
