LOCUS       sequence-166046-         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cas9 protein, under the control of the TEF1 promoter, and 
            CYC terminator. KanMX resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Jessop-Fabre MM, Jakociunas T, Stovicek V, Dai Z, Jensen MK, 
            Keasling JD, Borodina I
  TITLE     EasyClone-MarkerFree: A vector toolkit for marker-less integration 
            of genes into Saccharomyces cerevisiae via CRISPR-Cas9.
  JOURNAL   Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. 
            Epub 2016 Jun 23.
  PUBMED    27166612
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/biot.201600147"; journalName: "Biotechnol J"; date: 
            "2016-08"; volume: "11"; issue: "8"; pages: "1110-7"
COMMENT     Sequence Label: pCfB2312 (TEF1p-Cas9-CYC1t_kanMX)
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     269..285
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     269..285
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 attacccgta ctaaaggttt ggaaaagaaa aaagagaccg cctcgtttct ttttcttcgt
       61 cgaaaaaggc aataaaaatt tttatcacgt ttctttttct tgaaaatttt ttttttgatt
      121 tttttctctt tcgatgacct cccattgata tttaagttaa taaacggtct tcaatttctc
      181 aagtttcagt ttcatttttc ttgttctatt acaacttttt ttacttcttg ctcattagaa
      241 agaaagcata gcaatctaat ctaagttttc tagaactagt ggatcccccg ggaaaaatgg
      301 acaagaagta ctccattggg ctcgatatcg gcacaaacag cgtcggttgg gccgtcatta
      361 cggacgagta caaggtgccg agcaaaaaat tcaaagttct gggcaatacc gatcgccaca
      421 gcataaagaa gaacctcatt ggcgccctcc tgttcgactc cggggagacg gccgaagcca
      481 cgcggctcaa aagaacagca cggcgcagat atacccgcag aaagaatcgg atctgctacc
      541 tgcaggagat ctttagtaat gagatggcta aggtggatga ctctttcttc cataggctgg
      601 aggagtcctt tttggtggag gaggataaaa agcacgagcg ccacccaatc tttggcaata
      661 tcgtggacga ggtggcgtac catgaaaagt acccaaccat atatcatctg aggaagaagc
      721 ttgtagacag tactgataag gctgacttgc ggttgatcta tctcgcgctg gcgcatatga
      781 tcaaatttcg gggacacttc ctcatcgagg gggacctgaa cccagacaac ag
//
