LOCUS       sequence-16605-e        1397 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 1397)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD89_17
FEATURES             Location/Qualifiers
     source          1..1397
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             146..166
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(256..278)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 anngngnctg cnggtcgact ctagaggatc cccgnggatt ggccaaagga cccaaaggta
       61 tgtttcgaat gatactaaca taacatagaa cattttcagg aggacccttg cttggagggt
      121 accgagctca gaaaaaatga ctgctccaaa gaagaagcgt aaggtaccgg tgggtgaaga
      181 ccagaaacag cacctcgaac tgagccgcga tattgcccag cgtttcaacg cgctgtatgg
      241 cgagatcgat cccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
      301 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
      361 cgatcgccct tcccaacagt tgcgaaggta agtttaaaca gatccatact aactaacttg
      421 ttctgacata attttcagct tgaatggcga atggcgcttt gcctggtttc cggcaccaga
      481 agcggtgccg gaaagctggc tggagtgcga tcttcctgag gccgatactg tcgtcgtccc
      541 ctcaaactgg cagatgcacg gttacgatgc gcccatctac accaacgtga cctatcccat
      601 tacggtcaat ccgccgtttg ttcccacgga gaatccgacg ggttgttact cgctcacatt
      661 taatgttgat gaaagctggc tacaggaagg ccagacgcga attatttttg atggcgttaa
      721 ctcggcgttt catctgtggt gcaacgggcg ctgggtcggt tacggccagg taagtttaat
      781 taagttgata ctaactaaca agatctgatt aattttcagg acagtcgttt gccgtctgaa
      841 tttgacctga gcgcattttt acgcgccgga gaaaaccgcc tcgcggtgat ggtgctgcgc
      901 tggagtgacg gcagttatct ggaagatcag gatatgtggc ggatgagcgg cattttccgt
      961 gacgtctcgt tgctgcataa accgactaca caaatcagcg atttcatgnt gccnctcgct
     1021 ttatgatgat ttctcccgcg ctgtactgga ggctgaagtc caggtaagtt taaacaggat
     1081 ctnactaact aacatgctaa cactgaattt tcnanggggg ggagntgcgn gactacntac
     1141 gggtanagtt tcttntggga gggggaaanc aggtcccngn ggnacngncc ttttnggggg
     1201 naaatancna anangggggg gttnncccat cccccccnct acctngnang ncnaaanccn
     1261 aantgggngg cnnaanccca atttttnngg gggggtnaat nccccccccn ccccntntna
     1321 aaaaaacccg tttttttccn ggggnannaa ntttttttta aaancttttn nnggtnnccc
     1381 caattcttgg gggggaa
//
