LOCUS       sequence-166051-        1013 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of Gli1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1013)
  TITLE     Fernandez-Zapico lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: hGli1 flag3x
FEATURES             Location/Qualifiers
     source          1..1013
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(190..255)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 tgtcttccaa cttgcccctt gctccatacc acccccctcc accccataat attatagaag
       61 gacacctagt cagacaaaat gatgcaactt aattttatta ggacaaggct ggtgggcact
      121 ggagtggcaa cttccagggc caggagaggc actggggagg ggtcacaggg atgccacccg
      181 ggatcactac ttgtcatcgt catccttgta gtcgatgtca tgatctttat aatcaccgtc
      241 atggtctttg tagtcagccc gggatcctct agaggcacta gagttgagga attctgtttc
      301 cccaggtagg gatctcagta agacactcat gttgcccaca gccatgttgg ggggcccaga
      361 gggaggtggg gtatgtccag agctgccccg ctgatcatgg gaaggaggag gactcagccc
      421 ctggggctca tccagaatag ccacaaagtc cagctgagtg ttgtcaagat caagagagtc
      481 cagggggtta catacctgtc cttcgggagg agggtacaag gcaggacccc ctcctagcct
      541 gcccacctca ggatgaccac agctggggtt tgtgcctccc actttgagag gcccatagca
      601 agtgggcaat gggggcagaa gtcgaggtgg tgctgctgcc ctatgtgaag ccctatttgc
      661 ccccactaca aaattttcat ggcatggtga aggggtggga taggaacctg acttgtgatt
      721 gggcaagttg ggtccaaagc caggtccata tgtgttcact ggagctttag cacggcttgg
      781 gctaacctgg gaacccccca gaaacttggg actctggtag gaaggttcct gggcgggggc
      841 atcttccctg cccccaccct cccacagtag ctcctgttga gattgaacat aattacacac
      901 aagctgagcc ttgagctgcc ctgtggaatg ggtgggggaa tcaaagtcca ggcaaggcct
      961 gggttctgaa ggaagataat cagggggtgg ctgggtatag gggcctgact gga
//
