LOCUS       sequence-166054-         975 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Drosophila P-element transgenesis vector for cloning rescue 
            transgenes. Cloning site is flanked by FRT sequences and contains 
            constitutive RFP reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Zhou Q, Neal SJ, Pignoni F
  TITLE     Mutant Analysis by Rescue Gene Excision: New tools for mosaic 
            studies in Drosophila.
  JOURNAL   Genesis. 2016 Oct 3. doi: 10.1002/dvg.22984.
  PUBMED    27696669
REFERENCE   2  (bases 1 to 975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genesis. 
            2016 Oct 3. doi: 10.1002/dvg.22984."
COMMENT     Sequence Label: FoRC.PRa
FEATURES             Location/Qualifiers
     source          1..975
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(52..154)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     protein_bind    complement(299..530)
                     /gene="mutant version of attP"
                     /label=attP2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(548..567)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(549..567)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(572..588)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(572..588)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc
       61 cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt
      121 atggtgaaag ttggaacctc ttacgtgccg atcaacgtct cattttcgcc aaaagttggc
      181 ccagggcttc ccggtatcaa cagggacacc aggatttatt tattctgcga agtgatcttc
      241 cgtcacaggt atttattcgg cgcaaagtgc gtcgggtgat gctgccaact tagtcgacta
      301 caggtcacta ataccatcta agtagttgat tcatagtgac tggatatgtt gtgttttaca
      361 gtattatgta gtctgttttt tatgcaaagt ctaatttaat atattgatat ttatatcatt
      421 ttacgtttct cgttcagctt tcttgtacaa agttggcatt ataagaaagc attgcttatc
      481 aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc
      541 tgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc ggccggccac
      601 cgcggccgca ggtcgacaaa ggttaagcaa ttggctgacc atttaatttt ccacaagtcc
      661 aattcaagga caaggaaaac agaataagta cataagaccc cttgaaagtt aacgacaccc
      721 aacaaaagcc ggtttactta aatgcaaata catttgcaga ttcgaagcta aaaagggaaa
      781 aaaaaaccaa caaaaactcg acagctgact tcttttcgtg tatttgtatt tattgtattc
      841 gtttattcac aaatgaaatg caaaattctc ccgagtgttg cgcctgttct tgttacaaaa
      901 agtataaact caaagggagc aatgaatcct ccagttgttt agcctaatta tacacactaa
      961 aattttgttt ttttt
//
