LOCUS       sequence-166055-         894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Drosophila P-element transgenesis vector for cloning rescue 
            transgenes. Cloning site is flanked by FRT sequences and contains 
            constitutive RFP reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Zhou Q, Neal SJ, Pignoni F
  TITLE     Mutant Analysis by Rescue Gene Excision: New tools for mosaic 
            studies in Drosophila.
  JOURNAL   Genesis. 2016 Oct 3. doi: 10.1002/dvg.22984.
  PUBMED    27696669
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genesis. 
            2016 Oct 3. doi: 10.1002/dvg.22984."
COMMENT     Sequence Label: FoRC.PRa
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     691..707
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     691..707
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     712..731
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        712..730
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    748..847
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 atcgtggagc agtacgagcg cgccgagggc cgccaccacc tgttcctgta caagagcagg
       61 cacagaaggc atcgccagcg ctctaggagc cgcaatcgca gccgaagtcg cagcagtgaa
      121 cgaaaacgcc gtcaacggag ccgaagtcgc agcagtgaac gaagacgcta cttgtacagc
      181 ggccggccgc gactctaggc gatcgcgcgc cggtcgctac cattaccagt tggtctggtg
      241 tcaaaaataa taataaccgg gcagggggga tcgatccccc agatccgtac gatcctctag
      301 taaaaaaaaa acaaaatttt agtgtgtata attaggctaa acaactggag gattcattgc
      361 tccctttgag tttatacttt ttgtaacaag aacaggcgca acactcggga gaattttgca
      421 tttcatttgt gaataaacga atacaataaa tacaaataca cgaaaagaag tcagctgtcg
      481 agtttttgtt ggtttttttt tcccttttta gcttcgaatc tgcaaatgta tttgcattta
      541 agtaaaccgg cttttgttgg gtgtcgttaa ctttcaaggg gtcttatgta cttattctgt
      601 tttccttgtc cttgaattgg acttgtggaa aattaaatgg tcagccaatt gcttaacctt
      661 tgtcgacctg cggccgcggt ggccggccgc caggaaacag ctatgaccat gtaatacgac
      721 tcactatagg ggatatcagc tggatggcaa ataatgattt tattttgact gatagtgacc
      781 tgttcgttgc aacaaattga taagcaatgc tttcttataa tgccaacttt gtacaagaaa
      841 gctgaacgag aaacgtaaaa tgatataaat atcaatatat taaattagac tttg
//
