LOCUS       sequence-166058-         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of Gli1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  TITLE     Fernandez-Zapico lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: hGli1 6x his
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             123..140
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             144..176
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(180..188,189..203)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agctggctag cgtttaaact taagcttacc atggggggtt
      121 ctcatcatca tcatcatcat ggtatggcta gcatgactgg tggacagcaa atgggtcggg
      181 atctgtacga cgatgacgat aaggtaccag gatccagtgt ggtggaattc tgcagatatc
      241 cagcacagtg gcggccgcaa atgttcaact cgatgacccc accaccaatc agtagctatg
      301 gcgagccctg ctgtctccgg cccctcccca gtcagggggc ccccagtgtg gggacagaag
      361 gactgtctgg cccgcccttc tgccaccaag ctaacctcat gtccggcccc cacagttatg
      421 ggccagccag agagaccaac agctgcaccg agggcccact cttttcttct ccccggagtg
      481 cagtcaagtt gaccaagaag cgggcactgt ccatctcacc tctgtcggat gccagcctgg
      541 acctgcagac ggttatccgc acctcaccca gctccctcgt agctttcatc aactcgcgat
      601 gcacatctcc aggaggctcc tacggtcatc tctccattgg caccatgagc ccatctctgg
      661 gattcccagc ccagatgaat caccaaaaag ggccctcgcc ttcctttggg gtccagcctt
      721 gtggtcccca tgactctgcc cggggtggga tgatcccaca tcctcagtcc cggggaccct
      781 tcccaacttg ccagctgaag tctgagctgg acatgctggt tggcaagtgc cgggaggaac
      841 ccttggaagg tgatatgtcc agccccaact ccacaggcat acaggatccc ctgttgggga
      901 tgctggatgg gcgggaggac ctc
//
