LOCUS       sequence-16606-e        1305 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 1305)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD89_20
FEATURES             Location/Qualifiers
     source          1..1305
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             104..124
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(214..236)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gttggcaaag gacccaaagg tatgtttcga atgatactaa cataacatag aacattttca
       61 ggaggaccct tggagggtac cgagctcaga aaaaatgact gctccaaaga agaagcgtaa
      121 ggtaccggtg ggtgaagacc agaaacagca cctcgaactg agccgcgata ttgcccagcg
      181 tttcaacgcg ctgtatggcg agatcgatcc cgtcgtttta caacgtcgtg actgggaaaa
      241 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
      301 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgaaggtaag tttaaacaga
      361 tccatactaa ctaacttgtt ctgacataat tttcagcttg aatggcgaat ggcgctttgc
      421 ctggtttccg gcaccagaag cggtgccgga aagctggctg gagtgcgatc ttcctgaggc
      481 cgatactgtc gtcgtcccct caaactggca gatgcacggt tacgatgcgc ccatctacac
      541 caacgtgacc tatcccatta cggtcaatcc gccgtttgtt cccacggaga atccgacggg
      601 ttgttactcg ctcacattta atgttgatga aagctggcta caggaaggcc agacgcgaat
      661 tatttttgat ggcgttaact cggcgtttca tctgtggtgc aacgggcgct gggtcggtta
      721 cggccaggta agtttaatta agttgatact aactaacaag atctgattaa ttttcaggac
      781 agtcgtttgc cgtctgaatt tgacctgagc gcatttttac gcgccggaga aaaccgcctc
      841 gcggtgatgg tgctgcgctg gagtgacggc agttatctgg aagatcagga tatgtggcgg
      901 atgagcggca ttttccgtga cgtctcgttg ctgcataaac cgactacacn aatcagcgat
      961 ttccatgntg ccactcgctt taatgatgat ttctcccgcg ctgtactgga aggctgaagt
     1021 ccaggtaagt ttaaacagga tcttactaac taacatgcta acactgaatt ttcanatggg
     1081 cggngagttg cgtgactacc tacgggtaac nntttctttn tggcagggtg aaanccangn
     1141 tcccnagngg aacnggncnt tttgggggga aattntccaa aaacgggggg gntnngccna
     1201 tccctcccnt agntgaaagt caaaaaccna aatggggagg gccaaatccn aatttttnng
     1261 ggggggnnaa ccccccccca nnncccttnt naaaaaaacn ggtgt
//
