LOCUS       sequence-166060-        3588 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of Gli1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3588)
  TITLE     Fernandez-Zapico lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: hGli1 6x his
FEATURES             Location/Qualifiers
     source          1..3588
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             53..70
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             74..106
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(110..118,119..133)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     complement(3543..3560)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 ggggccccca agctggctag cgtttaaact taagcttacc atggggggtt ctcatcatca
       61 tcatcatcat ggtatggcta gcatgactgg tggacagcaa atgggtcggg atctgtacga
      121 cgatgacgat aaggtaccag gatccagtgt ggtggaattc tgcagatatc cagcacagtg
      181 gcggccgcaa atgttcaact cgatgacccc accaccaatc agtagctatg gcgagccctg
      241 ctgtctccgg cccctcccca gtcagggggc ccccagtgtg gggacagaag gactgtctgg
      301 cccgcccttc tgccaccaag ctaacctcat gtccggcccc cacagttatg ggccagccag
      361 agagaccaac agctgcaccg agggcccact cttttcttct ccccggagtg cagtcaagtt
      421 gaccaagaag cgggcactgt ccatctcacc tctgtcggat gccagcctgg acctgcagac
      481 ggttatccgc acctcaccca gctccctcgt agctttcatc aactcgcgat gcacatctcc
      541 aggaggctcc tacggtcatc tctccattgg caccatgagc ccatctctgg gattcccagc
      601 ccagatgaat caccaaaaag ggccctcgcc ttcctttggg gtccagcctt gtggtcccca
      661 tgactctgcc cggggtggga tgatcccaca tcctcagtcc cggggaccct tcccaacttg
      721 ccagctgaag tctgagctgg acatgctggt tggcaagtgc cgggaggaac ccttggaagg
      781 tgatatgtcc agccccaact ccacaggcat acaggatccc ctgttgggga tgctggatgg
      841 gcgggaggac ctcgagagag aggagaagcg tgagcctgaa tctgtgtatg aaactgactg
      901 ccgttgggat ggctgcagcc aggaatttga ctcccaagag cagctggtgc accacatcaa
      961 cagcgagcac atccacgggg agcggaagga gttcgtgtgc cactgggggg gctgctccag
     1021 ggagctgagg cccttcaaag cccagtacat gctggtggtt cacatgcgca gacacactgg
     1081 cgagaagcca cacaagtgca cgtttgaagg gtgccggaag tcatactcac gcctcgaaaa
     1141 cctgaagacg cacctgcggt cacacacggg tgagaagcca tacatgtgtg agcacgaggg
     1201 ctgcagtaaa gccttcagca atgccagtga ccgagccaag caccagaatc ggacccattc
     1261 caatgagaag ccgtatgtat gtaagctccc tggctgcacc aaacgctata cagatcctag
     1321 ctcgctgcga aaacatgtca agacagtgca tggtcctgac gcccatgtga ccaaacggca
     1381 ccgtggggat ggccccctgc ctcgggcacc atccatttct acagtggagc ccaagaggga
     1441 gcgggaagga ggtcccatca gggaggaaag cagactgact gtgccagagg gtgccatgaa
     1501 gccacagcca agccctgggg cccagtcatc ctgcagcagt gaccactccc cggcagggag
     1561 tgcagccaat acagacagtg gtgtggaaat gactggcaat gcagggggca gcactgaaga
     1621 cctctccagc ttggacgagg gaccttgcat tgctggcact ggtctgtcca ctcttcgccg
     1681 ccttgagaac ctcaggctgg accagctaca tcaactccgg ccaataggga cccggggtct
     1741 caaactgccc agcttgtccc acaccggtac cactgtgtcc cgccgcgtgg gccccccagt
     1801 ctctcttgaa cgccgcagca gcagctccag cagcatcagc tctgcctata ctgtcagccg
     1861 ccgctcctcc ctggcctctc ctttcccccc tggctcccca ccagagaatg gagcatcctc
     1921 cctgcctggc cttatgcctg cccagcacta cctgcttcgg gcaagatatg cttcagccag
     1981 agggggtggt acttcgccca ctgcagcatc cagcctggat cggataggtg gtcttcccat
     2041 gcctccttgg agaagccgag ccgagtatcc aggatacaac cccaatgcag gggtcacccg
     2101 gagggccagt gacccagccc aggctgctga ccgtcctgct ccagctagag tccagaggtt
     2161 caagagcctg ggctgtgtcc ataccccacc cactgtggca gggggaggac agaactttga
     2221 tccttacctc ccaacctctg tctactcacc acagcccccc agcatcactg agaatgctgc
     2281 catggatgct agagggctac aggaagagcc agaagttggg acctccatgg tgggcagtgg
     2341 tctgaacccc tatatggact tcccacctac tgatactctg ggatatgggg gacctgaagg
     2401 ggcagcagct gagccttatg gagcgagggg tccaggctct ctgcctcttg ggcctggtcc
     2461 acccaccaac tatggcccca acccctgtcc ccagcaggcc tcatatcctg accccaccca
     2521 agaaacatgg ggtgagttcc cttcccactc tgggctgtac ccaggcccca aggctctagg
     2581 tggaacctac agccagtgtc ctcgacttga acattatgga caagtgcaag tcaagccaga
     2641 acaggggtgc ccagtggggt ctgactccac aggactggca ccctgcctca atgcccaccc
     2701 cagtgagggg cccccacatc cacagcctct cttttcccat tacccccagc cctctcctcc
     2761 ccaatatctc cagtcaggcc cctataccca gccaccccct gattatcttc cttcagaacc
     2821 caggccttgc ctggactttg attcccccac ccattccaca gggcagctca aggctcagct
     2881 tgtgtgtaat tatgttcaat ctcaacagga gctactgtgg gagggtgggg gcagggaaga
     2941 tgcccccgcc caggaacctt cctaccagag tcccaagttt ctggggggtt cccaggttag
     3001 cccaagccgt gctaaagctc cagtgaacac atatggacct ggctttggac ccaacttgcc
     3061 caatcacaag tcaggttcct atcccacccc ttcaccatgc catgaaaatt ttgtagtggg
     3121 ggcaaatagg gcttcacata gggcagcagc accacctcga cttctgcccc cattgcccac
     3181 ttgctatggg cctctcaaag tgggaggcac aaaccccagc tgtggtcatc ctgaggtggg
     3241 caggctagga gggggtcctg ccttgtaccc tcctcccgaa ggacaggtat gtaaccccct
     3301 ggactctctt gatcttgaca acactcagct ggactttgtg gctattctgg atgagcccca
     3361 ggggctgagt cctcctcctt cccatgatca gcggggcagc tctggacata ccccacctcc
     3421 ctctgggccc cccaacatgg ctgtgggcaa catgagtgtc ttactgagat ccctacctgg
     3481 ggaaacagaa ttcctcaact ctagtgccta atctagaggg cccgtttaaa cccgctgatc
     3541 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctc
//
