LOCUS       sequence-166062-         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone-MarkerFree backbone vector for the addition of 1 or 2 
            genes and promoters for integration into site XI-1 (Chr XI: 
            67491..68573).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Jessop-Fabre MM, Jakociunas T, Stovicek V, Dai Z, Jensen MK, 
            Keasling JD, Borodina I
  TITLE     EasyClone-MarkerFree: A vector toolkit for marker-less integration 
            of genes into Saccharomyces cerevisiae via CRISPR-Cas9.
  JOURNAL   Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. 
            Epub 2016 Jun 23.
  PUBMED    27166612
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/biot.201600147"; journalName: "Biotechnol J"; date: 
            "2016-08"; volume: "11"; issue: "8"; pages: "1110-7"
COMMENT     Sequence Label: pCfB3036(XI-1 MarkerFree)
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    217..250
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     914..936
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 tctattattt atgatttttt gtatatacaa tatttctagt ttgttaaaga gaattaagaa
       61 aataaatctc gaaaataata aagggaaaat cagtttttga tatcaaaatt atacatgtca
      121 acgataatac aaaatataat acaaactata agatgttatc agtatttatt atgcatttag
      181 aataaatttt gtgtcgccct tattcgactc actatagaag ttcctattct ctagaaagta
      241 taggaacttc acttcatttt atttaaattt gcggccgcgc tgaggtctta atccacaccc
      301 acctcaaacc cattccttaa gagccgagtc cccatcaggt caatggtaga tatagccgcc
      361 catccttccc caacggccac ggttttagcg tcgtcgcctc cgccgcctcc tccggcgcac
      421 tcatgtgcca gcggcgaggc cctatttaca cttagggaaa ccccgccgcc ccaactagca
      481 accctcacgc ttagcgaaga acctcccgca attcccgcaa cacccgcacc ttccgctcgc
      541 gttcgtggcc actccccgca cagggtagtg catcaccatc taaacattgt caacagcttg
      601 gtccatagtt ctagcgcggc gtcatctcag gttccggctt cgacctagcc agaatgtgca
      661 gcagagtaaa taatattcac ttccctgttc ctaaaacgta caatatacaa caccaacctc
      721 atatcaacta ccagaaacag tatcatacgt aatagttatt ttttattttt ttaatttttt
      781 ttttttttgc cactgaccag ttttcgcggc ggtgtgagtt tccgtctgta cgcagcattt
      841 agcagagatt tgccaatgcc aagaaactat taatgcctca gcactagtcc tgcaggggta
      901 acgccagggt tttcccagtc acgacgttgt aaaacgacgg c
//
