LOCUS       sequence-166063-         998 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone-MarkerFree backbone vector for the addition of 1 or 2 
            genes and promoters for integration into site XI-5 (Chr XI: 
            11779..118967).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Jessop-Fabre MM, Jakociunas T, Stovicek V, Dai Z, Jensen MK, 
            Keasling JD, Borodina I
  TITLE     EasyClone-MarkerFree: A vector toolkit for marker-less integration 
            of genes into Saccharomyces cerevisiae via CRISPR-Cas9.
  JOURNAL   Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. 
            Epub 2016 Jun 23.
  PUBMED    27166612
REFERENCE   2  (bases 1 to 998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/biot.201600147"; journalName: "Biotechnol J"; date: 
            "2016-08"; volume: "11"; issue: "8"; pages: "1110-7"
COMMENT     Sequence Label: pCfB3037(XI-5 MarkerFree)
FEATURES             Location/Qualifiers
     source          1..998
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    179..212
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 ccacctgacc ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg
       61 actccccatg cgagagtagg gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa
      121 agactgggcc tttcgcccgg gctaattatg gggtgtcgcc cttattcgac tctatagtga
      181 agttcctatt ctctagaaag tataggaact tctgaagtgg ggatttaaat gcggccgcgc
      241 tgagggttta atgcggagaa gtcgttgata gcatttccga aggcttttcc acattgactg
      301 cagtatctaa aagagctcca gcagcggtct tagtagttcc tgtgcttgcc gctttgcagt
      361 atacagggac attgggaact acggaggaaa aatcggaatg ctctgagctc ttctcaaaac
      421 cagacgaact cgtcttattt ctcagtaaat tacccaacat ataaactctc cctcttcacc
      481 ttctctacaa ggccacacac aagacagttt taacaggcaa atggtactgg aggctctcac
      541 caatggggtt taagacgctc caaacacacg ctctcctttt tttctttata tatcggtagc
      601 aacgaaagct agtcgcagaa cgttacacac taaagcagat caatcaatca atcctgagaa
      661 cctgcttcgt tcaaaccatc cactagaaga aacacgcacg tgtacccaaa aatagagaca
      721 aacaaacaga caaaaataac aatgattcct accgactaaa gtaaaacaac actcacgtaa
      781 taaaaaatcc tagatacaac tcacttgtat tcctctcttc tttgaccatc actaaagctt
      841 ctctcttact atattcctca aataaaaaaa agttgcaatg attacttacc aatgtgccat
      901 aaactccgtg caccaattaa gtcctcagcg agctcgcatg gaatgcgtgc gatgagcgac
      961 ctcatgctat acctgagaaa gcaacctgac ctacagga
//
