LOCUS       sequence-166071-        1044 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Use to synthesize riboprobes for in situ hybridization (ISH) probe 
            for Alx1. Not for gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Parrilla M, Chang I, Degl'Innocenti A, Omura M
  TITLE     Expression of homeobox genes in the mouse olfactory epithelium.
  JOURNAL   J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. 
            Epub 2016 Jun 19.
  PUBMED    27243442
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.24051";
            journalName: "J Comp Neurol"; date: "2016-10-1- 1"; volume: "524"; 
            issue: "14"; pages: "2713-39"
COMMENT     Sequence Label: pISH-Alx1
FEATURES             Location/Qualifiers
     source          1..1044
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      953..962
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      957..966
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(996..1015)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(997..1015)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1022..1039)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1022..1038)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 atccaacgcg ttgggagctc tcccatatgg tcgacctgca ggcggccgcg aattcactag
       61 tgattaaatg cgtgtccgtt ggtagctcca gtgagaagag agtcagtgaa aaaattattg
      121 aggggcacgt ggctgaactg attctggtgg ttcgaaaacc ccgtataact ggaatctgtc
      181 cgaggcgagt gagagtaagg tgtcatgcag gaggacgcgt cacgtggtag catgcatgac
      241 gtaaccacag aaccaccgct agcatttcct gcccacaaat tgttctggat ctgtgggtag
      301 ctgtccgtcc ttggtaagac tgatatgtcg tacgtggcag cgaaatggct tttagcttgc
      361 tgtatttggc cgtatcgttc tctttttctc cacttggccc ttcgattttg aaaccaaacc
      421 tggaccctgg cctcagtgag ctccgttctc agggcaagtt gttctctgac atatacatct
      481 gggtaatggg ttttctggaa aactttctcc agttcctcca gctgcaaact ggtaaaggta
      541 gttcggtgtc tcctcttttt gctgctggat acattgctgt cacatttgtc cccaagttca
      601 tccagttcgc tcttctctgg catccctttc acgggagaca tcctgagatt gttgcagtta
      661 tccattgctc tattcagctc ggtgtgaagg ggctgtcctt ccactttggt gattccatag
      721 ttcacgctgc tgtcctgaca gggcgaagtc ctgtccaggc gcacgtgatg ctccgctcgc
      781 ggcagcggcc cgaaggcctg cacgcatttg ccagccgtcg ctttgccgta aaaggactca
      841 ttgtccagcg tctccataac gtgctccaac gcgcctcctg tgcccatgta aaagtcactg
      901 tttttacttg gcgggctctt cagggcaaac ttctcgcaat cgaattcccg cggccgccat
      961 ggcggccggg agcatgcgac gtcgggccca attcgcccta tagtgagtcg tattacaatt
     1021 cactggccgt cgttttacaa cgtc
//
