LOCUS       sequence-166072-        3889 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Use to synthesize riboprobes for in situ hybridization (ISH) probe 
            for Alx1. Not for gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3889)
  AUTHORS   Parrilla M, Chang I, Degl'Innocenti A, Omura M
  TITLE     Expression of homeobox genes in the mouse olfactory epithelium.
  JOURNAL   J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. 
            Epub 2016 Jun 19.
  PUBMED    27243442
REFERENCE   2  (bases 1 to 3889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.24051";
            journalName: "J Comp Neurol"; date: "2016-10-1- 1"; volume: "524"; 
            issue: "14"; pages: "2713-39"
COMMENT     Sequence Label: pISH-Alx1
FEATURES             Location/Qualifiers
     source          1..3889
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1014..1032)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1015..1032)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1050..1066)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1050..1066)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1063..1085)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1074..1090
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1098..1104,1105..1122,1123..1128))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1143..1164
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1281..1298)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1452..2040)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1532..1551)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2211..3002,3003..3071))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2834..2853
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3072..3176)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3254..3709)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3391..3412)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3603..3622
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3835..3857
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3849..3866
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3850..3866
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(3873..3889,1..3)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        join(3873..3889,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat
       61 tgcgagaagt ttgccctgaa gagcccgcca agtaaaaaca gtgactttta catgggcaca
      121 ggaggcgcgt tggagcacgt tatggagacg ctggacaatg agtcctttta cggcaaagcg
      181 acggctggca aatgcgtgca ggccttcggg ccgctgccgc gagcggagca tcacgtgcgc
      241 ctggacagga cttcgccctg tcaggacagc agcgtgaact atggaatcac caaagtggaa
      301 ggacagcccc ttcacaccga gctgaataga gcaatggata actgcaacaa tctcaggatg
      361 tctcccgtga aagggatgcc agagaagagc gaactggatg aacttgggga caaatgtgac
      421 agcaatgtat ccagcagcaa aaagaggaga caccgaacta cctttaccag tttgcagctg
      481 gaggaactgg agaaagtttt ccagaaaacc cattacccag atgtatatgt cagagaacaa
      541 cttgccctga gaacggagct cactgaggcc agggtccagg tttggtttca aaatcgaagg
      601 gccaagtgga gaaaaagaga acgatacggc caaatacagc aagctaaaag ccatttcgct
      661 gccacgtacg acatatcagt cttaccaagg acggacagct acccacagat ccagaacaat
      721 ttgtgggcag gaaatgctag cggtggttct gtggttacgt catgcatgct accacgtgac
      781 gcgtcctcct gcatgacacc ttactctcac tcgcctcgga cagattccag ttatacgggg
      841 ttttcgaacc accagaatca gttcagccac gtgcccctca ataatttttt cactgactct
      901 cttctcactg gagctaccaa cggacacgca tttaatcact agtgaattcg cggccgcctg
      961 caggtcgacc atatgggaga gctcccaacg cgttggatgc atagcttgag tattctatag
     1021 tgtcacctaa atagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     1081 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     1141 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     1201 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     1261 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     1321 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     1381 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     1441 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     1501 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     1561 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     1621 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     1681 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     1741 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     1801 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     1861 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     1921 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     1981 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     2041 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     2101 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     2161 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     2221 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     2281 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     2341 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     2401 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     2461 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     2521 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     2581 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     2641 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     2701 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     2761 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     2821 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     2881 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     2941 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     3001 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     3061 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     3121 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     3181 tttccccgaa aagtgccacc tgatgcggtg tgaaataccg cacagatgcg taaggagaaa
     3241 ataccgcatc aggaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
     3301 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     3361 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     3421 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     3481 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     3541 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     3601 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     3661 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc cattcgccat
     3721 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
     3781 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
     3841 cacgacgttg taaaacgacg gccagtgaat tgtaatacga ctcactata
//
