LOCUS       sequence-166076-        1012 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ISH probe for Cbr2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Ishii T, Omura M, Mombaerts P
  TITLE     Protocols for two- and three-color fluorescent RNA in situ 
            hybridization of the main and accessory olfactory epithelia in 
            mouse.
  JOURNAL   J Neurocytol. 2004 Dec;33(6):657-69. Epub 2005 Oct 11.
  PUBMED    16217621
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Neurocytol."; date: "2004-12"; volume: "33(6)"; pages: "657-69. Epub
            2005 Oct 11"
COMMENT     Sequence Label: pISH-Cbr2
FEATURES             Location/Qualifiers
     source          1..1012
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     5..21
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     6..22
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(547..565)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(586..602)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(586..602)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(599..621)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    610..626
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(634..640,641..658,659..664))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    679..700
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(817..834)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cccctcgagg tcgacggtat cgataagctt gatatcgaat tcgcagcatg aagctgaatt
       61 tcagtggcct gagggccctg gtgacagggg cagggaaagg gattggacgg gacactgtga
      121 aggccctgca cgcctcagga gccaaagtgg tggcggtgac tcggaccaac tcagacctgg
      181 tcagccttgc caaagagtgt cccggaatag agcccgtgtg tgtggacctg ggtgactggg
      241 atgccacaga gaaggcgctg ggcggtatcg gccctgtgga cttactggtg aacaatgcag
      301 cgctggttat aatgcagccc ttcctggaag ttaccaagga agcctttgac aggtccttca
      361 gtgtaaatct tcgctctgtg tttcaggtat cccagatggt agccagggac atgattaacc
      421 gtggcgtgcc agggtccatt gtcaatgtct ccagcatggt ggcccatgtc acctttccta
      481 acttgatcac ctacagctcc accatctaga gcggccgcca ccgcggtgga gctccagctt
      541 ttgttccctt tagtgagggt taatttcgag cttggcgtaa tcatggtcat agctgtttcc
      601 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
      661 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
      721 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
      781 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
      841 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
      901 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
      961 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg ac
//
