LOCUS       sequence-166077-         991 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at 
            site XII-5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Jessop-Fabre MM, Jakociunas T, Stovicek V, Dai Z, Jensen MK, 
            Keasling JD, Borodina I
  TITLE     EasyClone-MarkerFree: A vector toolkit for marker-less integration 
            of genes into Saccharomyces cerevisiae via CRISPR-Cas9.
  JOURNAL   Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. 
            Epub 2016 Jun 23.
  PUBMED    27166612
REFERENCE   2  (bases 1 to 991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/biot.201600147"; journalName: "Biotechnol J"; date: 
            "2016-08"; volume: "11"; issue: "8"; pages: "1110-7"
COMMENT     Sequence Label: pCfB3050(gRNA XII-5)
FEATURES             Location/Qualifiers
     source          1..991
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        146..489
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
       61 agacaagccc gtcagggcgc gtcagcgggc tgcatttaaa tgatctgttt agcttgcctc
      121 gtccccgccg ggtcacccgg ccagcgacat ggaggcccag aataccctcc ttgacagtct
      181 tgacgtgcgc agctcagggg catgatgtga ctgtcgcccg tacatttagc ccatacatcc
      241 ccatgtataa tcatttgcat ccatacattt tgatggccgc acggcgcgaa gcaaaaatta
      301 cggctcctcg ctgcagacct gcgagcaggg aaacgctccc ctcacagacg cgttgaattg
      361 tccccacgcc gcgcccctgt agagaaatat aaaaggttag gatttgccac tgaggttctt
      421 ctttcatata cttcctttta aaatcttgct aggatacagt tctcacatca catccgaaca
      481 taaacaacca tgggtaccac tcttgacgac acggcttacc ggtaccgcac cagtgtcccg
      541 ggggacgccg aggccatcga ggcactggat gggtccttca ccaccgacac cgttttccgc
      601 gtcaccacca ccggggacgg cttcaccctg cgggaggtgc cggtggaccc gcccctgacc
      661 aaggtgttcc ccgacgacga atcggacgac gaatcggacg acggggagga cggcgacccg
      721 gactcccgga cgttcgtcgc gtacggggac gacggcgacc tggcgggctt cgtggtcgtc
      781 tcgtactccg gctggaaccg ccggctgacc gtcgaggaca tcgaggtcgc cccggagcac
      841 cgggggcacg gggtcgggcg cgcgttgatg gggctcgcga cggagttcgc ccgcgagcgg
      901 ggcgccgggc acctctggct ggaggtcacc aacgtcaacg caccggcgat ccacgcgtac
      961 cggcggatgg ggttcaccct ctgcggcctg g
//
