LOCUS       sequence-166080-         995 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Use to synthesize riboprobes for in situ hybridization (ISH) probe 
            for Barx1. Not for gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Parrilla M, Chang I, Degl'Innocenti A, Omura M
  TITLE     Expression of homeobox genes in the mouse olfactory epithelium.
  JOURNAL   J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. 
            Epub 2016 Jun 19.
  PUBMED    27243442
REFERENCE   2  (bases 1 to 995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.24051";
            journalName: "J Comp Neurol"; date: "2016-10-1- 1"; volume: "524"; 
            issue: "14"; pages: "2713-39"
COMMENT     Sequence Label: pISH-Barx1
FEATURES             Location/Qualifiers
     source          1..995
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      12..21
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(537..555)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(538..555)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(573..589)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(573..589)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(586..608)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    597..613
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(621..627,628..645,646..651))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    666..687
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(804..821)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gctcccggcc gccatggcgg ccgcgggaat tcgattagac aattaagggc cagacaagga
       61 aggacacagg cccggaagcc aatcccaggt gtcagcgagc ttctgtcccc agtctgggag
      121 acttgtgtcc agcgtggccg aagcttctgg tctttctcgg acctccgcag tgtgcggcgc
      181 tccacgctca ttcacgcccc gctccttgcc cacactttta tccccggcct ccagccggcc
      241 ttctgggccc ggacaccggc aggcacacac ttacttccgc gccttgggga ccccgccacc
      301 gggtgcaggt ccctatggcc ctgccctgca gagcagagtc gtctctagca gatcagctga
      361 tacctggcct ccccatgtcg ctgaggcttc tacccctctc ttacaaagac ggtgactctt
      421 ttttccaata aaatatttta tgacacagtg ggggacaatc actagtgaat tcgcggccgc
      481 ctgcaggtcg accatatggg agagctccca acgcgttgga tgcatagctt gagtattcta
      541 tagtgtcacc taaatagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
      601 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
      661 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
      721 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
      781 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
      841 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
      901 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
      961 gcgttgctgg cgtttttcca taggctccgc ccccc
//
