LOCUS       sequence-166084-        1083 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Use to synthesize riboprobes for in situ hybridization (ISH) probe 
            for Barx1. Not for gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Parrilla M, Chang I, Degl'Innocenti A, Omura M
  TITLE     Expression of homeobox genes in the mouse olfactory epithelium.
  JOURNAL   J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. 
            Epub 2016 Jun 19.
  PUBMED    27243442
REFERENCE   2  (bases 1 to 1083)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.24051";
            journalName: "J Comp Neurol"; date: "2016-10-1- 1"; volume: "524"; 
            issue: "14"; pages: "2713-39"
COMMENT     Sequence Label: pISH-Barx1
FEATURES             Location/Qualifiers
     source          1..1083
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      501..510
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      505..514
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(544..563)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(545..563)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(570..587)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(570..586)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(579..601)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(814..833)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1024..1045
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 atccaacgcg ttgggagctc tcccatatgg tcgacctgca ggcggccgcg aattcactag
       61 tgattgtccc ccactgtgtc ataaaatatt ttattggaaa aaagagtcac cgtctttgta
      121 agagaggggt agaagcctca gcgacatggg gaggccaggt atcagctgat ctgctagaga
      181 cgactctgct ctgcagggca gggccatagg gacctgcacc cggtggcggg gtccccaagg
      241 cgcggaagta agtgtgtgcc tgccggtgtc cgggcccaga aggccggctg gaggccgggg
      301 ataaaagtgt gggcaaggag cggggcgtga atgagcgtgg agcgccgcac actgcggagg
      361 tccgagaaag accagaagct tcggccacgc tggacacaag tctcccagac tggggacaga
      421 agctcgctga cacctgggat tggcttccgg gcctgtgtcc ttccttgtct ggcccttaat
      481 tgtctaatcg aattcccgcg gccgccatgg cggccgggag catgcgacgt cgggcccaat
      541 tcgccctata gtgagtcgta ttacaattca ctggccgtcg ttttacaacg tcgtgactgg
      601 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
      661 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
      721 gaatggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
      781 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
      841 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
      901 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
      961 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     1021 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     1081 att
//
