LOCUS       sequence-166085-        3437 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Use to synthesize riboprobes for in situ hybridization (ISH) probe 
            for Barx1. Not for gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Parrilla M, Chang I, Degl'Innocenti A, Omura M
  TITLE     Expression of homeobox genes in the mouse olfactory epithelium.
  JOURNAL   J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. 
            Epub 2016 Jun 19.
  PUBMED    27243442
REFERENCE   2  (bases 1 to 3437)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.24051";
            journalName: "J Comp Neurol"; date: "2016-10-1- 1"; volume: "524"; 
            issue: "14"; pages: "2713-39"
COMMENT     Sequence Label: pISH-Barx1
FEATURES             Location/Qualifiers
     source          1..3437
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(562..580)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(563..580)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(598..614)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(598..614)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(611..633)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    622..638
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(646..652,653..670,671..676))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    691..712
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(829..846)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1000..1588)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1080..1099)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1759..2550,2551..2619))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2382..2401
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2620..2724)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2802..3257)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2939..2960)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3151..3170
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3383..3405
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3397..3414
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3398..3414
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(3421..3437,1..3)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        join(3421..3437,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat
       61 tagacaatta agggccagac aaggaaggac acaggcccgg aagccaatcc caggtgtcag
      121 cgagcttctg tccccagtct gggagacttg tgtccagcgt ggccgaagct tctggtcttt
      181 ctcggacctc cgcagtgtgc ggcgctccac gctcattcac gccccgctcc ttgcccacac
      241 ttttatcccc ggcctccagc cggccttctg ggcccggaca ccggcaggca cacacttact
      301 tccgcgcctt ggggaccccg ccaccgggtg caggtcccta tggccctgcc ctgcagagca
      361 gagtcgtctc tagcagatca gctgatacct ggcctcccca tgtcgctgag gcttctaccc
      421 ctctcttaca aagacggtga ctcttttttc caataaaata ttttatgaca cagtggggga
      481 caatcactag tgaattcgcg gccgcctgca ggtcgaccat atgggagagc tcccaacgcg
      541 ttggatgcat agcttgagta ttctatagtg tcacctaaat agcttggcgt aatcatggtc
      601 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
      661 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
      721 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
      781 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
      841 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
      901 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
      961 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     1021 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     1081 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     1141 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     1201 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     1261 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     1321 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     1381 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     1441 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     1501 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     1561 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     1621 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     1681 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     1741 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     1801 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     1861 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     1921 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     1981 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     2041 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     2101 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     2161 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     2221 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     2281 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     2341 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     2401 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     2461 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     2521 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     2581 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     2641 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     2701 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg atgcggtgtg
     2761 aaataccgca cagatgcgta aggagaaaat accgcatcag gaaattgtaa gcgttaatat
     2821 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     2881 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     2941 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     3001 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     3061 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     3121 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     3181 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     3241 gccgctacag ggcgcgtcca ttcgccattc aggctgcgca actgttggga agggcgatcg
     3301 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
     3361 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaattg
     3421 taatacgact cactata
//
