LOCUS       sequence-166090-        1043 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Use to synthesize riboprobes for in situ hybridization (ISH) probe 
            for Barx2. Not for gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Parrilla M, Chang I, Degl'Innocenti A, Omura M
  TITLE     Expression of homeobox genes in the mouse olfactory epithelium.
  JOURNAL   J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. 
            Epub 2016 Jun 19.
  PUBMED    27243442
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.24051";
            journalName: "J Comp Neurol"; date: "2016-10-1- 1"; volume: "524"; 
            issue: "14"; pages: "2713-39"
COMMENT     Sequence Label: pISH-Barx2
FEATURES             Location/Qualifiers
     source          1..1043
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      877..886
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      881..890
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(920..939)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(921..939)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(946..963)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(946..962)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(955..977)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 acgcgttggg agctctccca tatggtcgac ctgcaggcgg ccgcgaattc actagtgatt
       61 ggagggagaa gagcagccct tgctgcaggc cagcggagct gctgtagcca gccctgcagc
      121 tatcacccaa gcctgggcaa tcagatccaa ggcgctatct cccttacaga agggtatctg
      181 tgcctaacac agccttgcaa gaaggttaag tcccacacaa tcgagtcttc atcaaatgct
      241 tccgatgaaa atgacgctgg gacgtgggag accagaagtg ctcctctacc cacgttacta
      301 gtctttagag gtcttctctc cctcattctc cttgcctctc tcttcccttc ttcccagctc
      361 ccccttcccc tcaaagaact ttattttagc ttaatggtgg gggttcagag gaagcttctg
      421 ataactcctg gggctggtga ataggttctg ccacctccaa ggggacaagg cacgctttgg
      481 gctcctgggt atcacagggc tcctcctgtc tctccgagga ttccagcagc tcctggctct
      541 gagcctggct gttcatcttc tcttcagctt caatctcctc tgatgtggga atggaattct
      601 tcttagggcg ccccttaggt tttgtgggtg cttcctgtcc acctttaagg accatcttct
      661 tccatttcat cctgcgattc tgataccaag tcttcacttg cagctgagtg agtcccagag
      721 actgggccaa gtccaacctg tctggggtag acaaatactt ctgcttctgg aatttcttct
      781 ctaggcccat gagctgcagc tcggtgaaga tggtgcgact tctgcgtggt ttcttctgcc
      841 tgggcgtggg ctgttctgtc taatcgaatt cccgcggccg ccatggcggc cgggagcatg
      901 cgacgtcggg cccaattcgc cctatagtga gtcgtattac aattcactgg ccgtcgtttt
      961 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     1021 ccctttcgcc agctggcgta ata
//
