LOCUS       sequence-166091-        3818 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Use to synthesize riboprobes for in situ hybridization (ISH) probe 
            for Barx2. Not for gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3818)
  AUTHORS   Parrilla M, Chang I, Degl'Innocenti A, Omura M
  TITLE     Expression of homeobox genes in the mouse olfactory epithelium.
  JOURNAL   J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. 
            Epub 2016 Jun 19.
  PUBMED    27243442
REFERENCE   2  (bases 1 to 3818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.24051";
            journalName: "J Comp Neurol"; date: "2016-10-1- 1"; volume: "524"; 
            issue: "14"; pages: "2713-39"
COMMENT     Sequence Label: pISH-Barx2
FEATURES             Location/Qualifiers
     source          1..3818
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(943..961)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(944..961)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(979..995)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(979..995)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(992..1014)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1003..1019
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1027..1033,1034..1051,1052..1057))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1072..1093
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1210..1227)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1381..1969)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1461..1480)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2140..2931,2932..3000))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2763..2782
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3001..3105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3183..3638)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3320..3341)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3532..3551
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3764..3786
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3778..3795
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3779..3795
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(3802..3818,1..3)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        join(3802..3818,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat
       61 tagacagaac agcccacgcc caggcagaag aaaccacgca gaagtcgcac catcttcacc
      121 gagctgcagc tcatgggcct agagaagaaa ttccagaagc agaagtattt gtctacccca
      181 gacaggttgg acttggccca gtctctggga ctcactcagc tgcaagtgaa gacttggtat
      241 cagaatcgca ggatgaaatg gaagaagatg gtccttaaag gtggacagga agcacccaca
      301 aaacctaagg ggcgccctaa gaagaattcc attcccacat cagaggagat tgaagctgaa
      361 gagaagatga acagccaggc tcagagccag gagctgctgg aatcctcgga gagacaggag
      421 gagccctgtg atacccagga gcccaaagcg tgccttgtcc ccttggaggt ggcagaacct
      481 attcaccagc cccaggagtt atcagaagct tcctctgaac ccccaccatt aagctaaaat
      541 aaagttcttt gaggggaagg gggagctggg aagaagggaa gagagaggca aggagaatga
      601 gggagagaag acctctaaag actagtaacg tgggtagagg agcacttctg gtctcccacg
      661 tcccagcgtc attttcatcg gaagcatttg atgaagactc gattgtgtgg gacttaacct
      721 tcttgcaagg ctgtgttagg cacagatacc cttctgtaag ggagatagcg ccttggatct
      781 gattgcccag gcttgggtga tagctgcagg gctggctaca gcagctccgc tggcctgcag
      841 caagggctgc tcttctccct ccaatcacta gtgaattcgc ggccgcctgc aggtcgacca
      901 tatgggagag ctcccaacgc gttggatgca tagcttgagt attctatagt gtcacctaaa
      961 tagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     1021 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     1081 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     1141 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     1201 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     1261 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     1321 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     1381 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     1441 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     1501 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     1561 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     1621 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     1681 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     1741 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     1801 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     1861 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     1921 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     1981 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     2041 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     2101 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     2161 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     2221 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     2281 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     2341 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     2401 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     2461 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     2521 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     2581 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     2641 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     2701 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     2761 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     2821 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     2881 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     2941 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     3001 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     3061 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     3121 agtgccacct gatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     3181 ggaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     3241 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
     3301 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     3361 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
     3421 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
     3481 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
     3541 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
     3601 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc attcgccatt caggctgcgc
     3661 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
     3721 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
     3781 aaaacgacgg ccagtgaatt gtaatacgac tcactata
//
