LOCUS       sequence-166096-        1001 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Example plasmid containing a bidirectional promoter of pTDH3 and 
            pTEF1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Jessop-Fabre MM, Jakociunas T, Stovicek V, Dai Z, Jensen MK, 
            Keasling JD, Borodina I
  TITLE     EasyClone-MarkerFree: A vector toolkit for marker-less integration 
            of genes into Saccharomyces cerevisiae via CRISPR-Cas9.
  JOURNAL   Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. 
            Epub 2016 Jun 23.
  PUBMED    27166612
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/biot.201600147"; journalName: "Biotechnol J"; date: 
            "2016-08"; volume: "11"; issue: "8"; pages: "1110-7"
COMMENT     Sequence Label: p1977 (pUC19-PTDH3-PTEF1)
FEATURES             Location/Qualifiers
     source          1..1001
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     170..192
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     184..201
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     185..201
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(230..896)
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     complement(337..360)
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
ORIGIN
        1 tgaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg
       61 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
      121 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
      181 cgttgtaaaa cgacggccag tgaattcgag ctcggtaccc acctgcactt ttgtttgttt
      241 atgtgtgttt attcgaaact aagttcttgg tgttttaaaa ctaaaaaaaa gactaactat
      301 aaaagtagaa tttaagaagt ttaagaaata gatttacaga attacaatca atacctaccg
      361 tctttatata cttattagtc aagtagggga ataatttcag ggaactggtt tcaacctttt
      421 ttttcagctt tttccaaatc agagagagca gaaggtaata gaaggtgtaa gaaaatgaga
      481 tagatacatg cgcgggtcaa ttgccttgtg tcatcattta ctccaggcag gttgcatcac
      541 tccattgagg ttgtgcccgt tttttgcctg tttgtgcccc tgttctctgt agttgcgcta
      601 agagaatgga cctatgaact gatggttggt gaagaaaaca atattttggt gctgggattc
      661 tttttttttc tggatgccag cttaaaaagc gggctccatt atatttagtg gatgccagga
      721 ataaactgtt cacccagaca cctacgatgt tatatattct gtgtaacccg ccccctattt
      781 tgggcatgta cgggttacag cagaattaaa aggctaattt tttgactaaa taaagttagg
      841 aaaatcacta ctattaatta tttacgtatt ctttgaaatg gcagtattga taatgataaa
      901 ctcgaactga aaaagcgtgt tttttatagc tactgatgca cacaccatag cttcaaaatg
      961 tttctactcc ttttttactc ttccagattt tctcggactc c
//
