LOCUS       sequence-166098-        1055 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  human Doublecortin, N-terminal 6-His tag, C-terminal EGFP-Strep-tag 
            II.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Bechstedt S, Brouhard GJ
  TITLE     Doublecortin recognizes the 13-protofilament microtubule 
            cooperatively and tracks microtubule ends.
  JOURNAL   Dev Cell. 2012 Jul 17;23(1):181-92. doi: 
            10.1016/j.devcel.2012.05.006. Epub 2012 Jun 21.
  PUBMED    22727374
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2012.05.006"; journalName: "Dev Cell"; date: 
            "2012-07-17- 17"; volume: "23"; issue: "1"; pages: "181-92"
COMMENT     Sequence Label: hsDCX-EGFP-pHAT
FEATURES             Location/Qualifiers
     source          1..1055
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             18..40
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             54..71
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..101
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 attcctctag caataatttt gtttaacttt aagaaggaga tatatccatg agtcatcacc
       61 atcaccatca ctccatgctg gaagttctgt tccaggggcc caccatgggt cgacagctga
      121 ggggagctat ggaacttgat tttggacact ttgacgaaag agataagaca tccaggaaca
      181 tgcgaggctc ccggatgaat gggttgccta gccccactca cagcgcccac tgtagcttct
      241 accgaaccag aaccttgcag gcactgagta atgagaagaa agccaagaag gtacgtttct
      301 accgcaatgg ggaccgctac ttcaagggga ttgtgtacgc tgtgtcctct gaccgttttc
      361 gcagctttga cgccttgctg gctgacctga cgcgatctct gtccgacaac atcaacctgc
      421 ctcagggagt gcgttacatt tacaccattg atggatccag gaagatcgga agcatggatg
      481 aactggagga aggggaaagc tatgtctgtt cctcagacaa cttctttaaa aaggtggagt
      541 acaccaagaa tgtcaatccc aactggtctg tcaacgtaaa aacatctgcc aatatgaaag
      601 ccccccagtc tttggctagc agcaacagtg cacaggccag ggagaacaag gactttgtgc
      661 gccccaagct ggttaccatc atccgcagtg gggtgaagcc tcggaaggct gtgcgtgtgc
      721 ttctgaacaa gaagacagcc cactcttttg agcaagtcct cactgatatc acagaagcca
      781 tcaaactgga gaccggggtt gtcaaaaaac tctacactct ggatggaaaa caggtaactt
      841 gtctccatga tttctttggt gatgatgatg tgtttattgc ctgtggtcct gaaaaatttc
      901 gctatgctca ggatgatttt tctctggatg aaaatgaatg ccgagtcatg aagggaaacc
      961 catcagccac agctggccca aaggcatccc caacacctca gaagacttca gccaagagcc
     1021 ctggtcctat gcgccgaagc aagtctccag ctgac
//
