LOCUS       sequence-166100-        1000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Empty vector, for the insertion of gRNA expression cassettes into 
            the vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Jessop-Fabre MM, Jakociunas T, Stovicek V, Dai Z, Jensen MK, 
            Keasling JD, Borodina I
  TITLE     EasyClone-MarkerFree: A vector toolkit for marker-less integration 
            of genes into Saccharomyces cerevisiae via CRISPR-Cas9.
  JOURNAL   Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. 
            Epub 2016 Jun 23.
  PUBMED    27166612
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/biot.201600147"; journalName: "Biotechnol J"; date: 
            "2016-08"; volume: "11"; issue: "8"; pages: "1110-7"
COMMENT     Sequence Label: pTAJAK-71 (pESC-NatMXsyn-USER)
FEATURES             Location/Qualifiers
     source          1..1000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     210..227
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(460..657)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
       61 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
      121 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
      181 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
      241 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggaatg
      301 cacgcgatcg cacgcattcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
      361 cccaacagtt gcgcagcctg aatggcgaat ggacgcgccc tgtaggcggc cgcagctcgt
      421 tttcgacact ggatggcggc gttagtatcg aatcgacagc agtatagcga ccagcattca
      481 catacgattg acgcatgata ttactttctg cgcacttaac ttcgcatctg ggcagatgat
      541 gtcgaggcga aaaaaaatat aaatcacgct aacatttgat taaaatagaa caactacaat
      601 ataaaaaaac tatacaaatg acaagttctt gaaaacaaga atctttttat tgtcagtact
      661 gattaggggc agggcatgct catgtagagc gcctgctcgc cgtccgaggc ggtgccgtcg
      721 tacagggcgg tgtccaggcc gcagagggtg aaccccatcc gccggtacgc gtggatcgcc
      781 ggtgcgttga cgttggtgac ctccagccag aggtgcccgg cgccccgctc gcgggcgaac
      841 tccgtcgcga gccccatcaa cgcgcgcccg accccgtgcc cccggtgctc cggggcgacc
      901 tcgatgtcct cgacggtcag ccggcggttc cagccggagt acgagacgac cacgaagccc
      961 gccaggtcgc cgtcgtcccc gtacgcgacg aacgtccggg
//
