LOCUS       sequence-166102-        3831 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Use to synthesize riboprobes for in situ hybridization (ISH) probe 
            for Dmbx1. Not for gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Parrilla M, Chang I, Degl'Innocenti A, Omura M
  TITLE     Expression of homeobox genes in the mouse olfactory epithelium.
  JOURNAL   J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. 
            Epub 2016 Jun 19.
  PUBMED    27243442
REFERENCE   2  (bases 1 to 3831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.24051";
            journalName: "J Comp Neurol"; date: "2016-10-1- 1"; volume: "524"; 
            issue: "14"; pages: "2713-39"
COMMENT     Sequence Label: pISH-Dmbx1
FEATURES             Location/Qualifiers
     source          1..3831
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(956..974)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(957..974)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(992..1008)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(992..1008)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1005..1027)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1016..1032
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1040..1046,1047..1064,1065..1070))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1085..1106
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1223..1240)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1394..1982)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1474..1493)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2153..2944,2945..3013))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2776..2795
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3014..3118)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3196..3651)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3333..3354)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3545..3564
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3777..3799
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3791..3808
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3792..3808
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(3815..3831,1..3)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        join(3815..3831,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat
       61 taaccatggg accgagacag gggtacaccc aagaccatgg ttgggttaga agccagacag
      121 tcaattgggg agtgtatcga gtcccaggga tgcggcgtgc tgcttggccc ggaggcgaag
      181 gttttcaatg ctcgtggttt tactgttcag ggcagcggag gcaggtgcca ggcctgtggg
      241 gggcgccggc agcaatgggg acccccacac accctggtgg gcagcagcag ctgctgacag
      301 ggattggtaa taggactggc agtgtaggga gctcaatggg gccatgttga cccctaggta
      361 gggcacggca gccgccgcag ctgcagccgc cgcaggtgcg ggaccgccca cttcaaaaga
      421 cgagtagtgc atcaggttgt tggtggccgc catatgctgg cggaactgct cctgcagacg
      481 gaagaggctg agtggggatg aggagtagga gtgggaagga ccaaggaggc cgccccctgg
      541 ggctgcgggc gttatggcca ggctgcctgg ggaatctgcc ttagggctgc ccctcttgca
      601 gcccggcacc ttgctctcag atccagggct cttttcagct ttgggctcct cggcacggga
      661 gtcctcttca cggtccagct gatcttctgg ggcagactcg ctggctgact gctccgacag
      721 gctcagttga agttcagcag gaggatcacc actgggcaaa ccaggaggct gttctgtttc
      781 cagctgcgtg tctgaggctg gggcctccac cttgccttct ccgtgggaac cctcggcctc
      841 cttctgtttc tggagctgct ctttctgcag gctgcaatca ctagtgaatt cgcggccgcc
      901 tgcaggtcga ccatatggga gagctcccaa cgcgttggat gcatagcttg agtattctat
      961 agtgtcacct aaatagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     1021 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     1081 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     1141 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     1201 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     1261 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     1321 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     1381 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     1441 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     1501 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     1561 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     1621 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     1681 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     1741 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     1801 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     1861 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     1921 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     1981 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     2041 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     2101 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     2161 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     2221 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     2281 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     2341 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     2401 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     2461 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     2521 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     2581 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     2641 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     2701 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     2761 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     2821 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     2881 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     2941 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     3001 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     3061 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     3121 catttccccg aaaagtgcca cctgatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     3181 aaataccgca tcaggaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
     3241 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     3301 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     3361 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     3421 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     3481 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
     3541 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
     3601 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tccattcgcc
     3661 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
     3721 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
     3781 gtcacgacgt tgtaaaacga cggccagtga attgtaatac gactcactat a
//
