LOCUS       sequence-166103-         947 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  human Doublecortin, N-terminal 6-His tag, C-terminal EGFP-Strep-tag 
            II.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Bechstedt S, Brouhard GJ
  TITLE     Doublecortin recognizes the 13-protofilament microtubule 
            cooperatively and tracks microtubule ends.
  JOURNAL   Dev Cell. 2012 Jul 17;23(1):181-92. doi: 
            10.1016/j.devcel.2012.05.006. Epub 2012 Jun 21.
  PUBMED    22727374
REFERENCE   2  (bases 1 to 947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2012.05.006"; journalName: "Dev Cell"; date: 
            "2012-07-17- 17"; volume: "23"; issue: "1"; pages: "181-92"
COMMENT     Sequence Label: hsDCX-EGFP-pHAT
FEATURES             Location/Qualifiers
     source          1..947
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        42..60
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     42..59
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             complement(76..99)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             complement(join(118..828,829..831,832..834))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(118..828,829..831,832..834))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(160..181)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     509..528
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     768..789
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 agcttccttt cgggctttgt tagcagccgg atcaagctgg gatttaggtg acactataga
       61 atactcaagc tttcattttt cgaactgcgg gtggctccag gccgcggccg cggatccctt
      121 gtacagctcg tccatgccga gagtgatccc ggcggcggtc acgaactcca gcaggaccat
      181 gtgatcgcgc ttctcgttgg ggtctttgct cagggcggac tgggtgctca ggtagtggtt
      241 gtcgggcagc agcacggggc cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag
      301 ctgcacgctg ccgtcctcga tgttgtggcg gatcttgaag ttcaccttga tgccgttctt
      361 ctgcttgtcg gccatgatat agacgttgtg gctgttgtag ttgtactcca gcttgtgccc
      421 caggatgttg ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt
      481 gtcgccctcg aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat
      541 ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg aagaagtcgt gctgcttcat
      601 gtggtcgggg tagcggctga agcactgcac gccgtaggtc agggtggtca cgagggtggg
      661 ccagggcacg ggcagcttgc cggtggtgca gatgaacttc agggtcagct tgccgtaggt
      721 ggcatcgccc tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt cgccgtccag
      781 ctcgaccagg atgggcacca ccccggtgaa cagctcctcg cccttgctca ccatttgctg
      841 aggggaactc atggaatcac caagcgagtc cgagtcatcc aaggacagag gcaggtacag
      901 gtccttgtgc ttccggaggc tgccaggact ggtgggcgta gagatgg
//
