LOCUS       sequence-166105-        1004 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  hsDCX-pHAT N-terminal 6xHis-tag, C-terminal Strep-tagII.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Bechstedt S, Brouhard GJ
  TITLE     Doublecortin recognizes the 13-protofilament microtubule 
            cooperatively and tracks microtubule ends.
  JOURNAL   Dev Cell. 2012 Jul 17;23(1):181-92. doi: 
            10.1016/j.devcel.2012.05.006. Epub 2012 Jun 21.
  PUBMED    22727374
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2012.05.006"; journalName: "Dev Cell"; date: 
            "2012-07-17- 17"; volume: "23"; issue: "1"; pages: "181-92"
COMMENT     Sequence Label: hsDCX-pHAT
FEATURES             Location/Qualifiers
     source          1..1004
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     291..312
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     terminator      complement(548..595)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     591..609
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     promoter        667..685
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     667..684
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             complement(701..724)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg
       61 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
      121 cgactggaaa gcgggcagtg agcgcaacgc aattaatgta agttagctca ctcattaggc
      181 accccaggct ttacacttta tgcttccgac ctgcaggcat gcaagctgtg ggccatcgcc
      241 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
      301 gttccaaact ggaacaacac tcaacactat ctcggtctat tcttttgatt tataagggat
      361 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
      421 ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg caactgttgg
      481 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagg ttatccggat atagttcctc
      541 ctttcagcaa aaaacccctc aagacccgtt tagaggcccc aaggggttat gctagttatt
      601 gctcagcggt ggcagcagcc aactcagctt cctttcgggc tttgttagca gccggatcaa
      661 gctgggattt aggtgacact atagaatact caagctttca tttttcgaac tgcgggtggc
      721 tccagggctg aggggaactc atggaatcac caagcgagtc cgagtcatcc aaggacagag
      781 gcaggtacag gtccttgtgc ttccggaggc tgccaggact ggtgggcgta gagatgggag
      841 actgcttaga cttgggggta gagagctggc tgctggaggt tccgtttgcg tcttggtcgt
      901 tacctgagtc agctggagac ttgcttcggc gcataggacc agggctcttg gctgaagtct
      961 tctgaggtgt tggggatgcc tttgggccag ctgtggctga tggg
//
