LOCUS       sequence-166107-         992 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  U6 telestem mutant (A34G/A35G) for in vivo studies.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Rodgers ML, Didychuk AL, Butcher SE, Brow DA, Hoskins AA
  TITLE     A multi-step model for facilitated unwinding of the yeast U4/U6 RNA 
            duplex.
  JOURNAL   Nucleic Acids Res. 2016 Dec 15;44(22):10912-10928. doi: 
            10.1093/nar/gkw686. Epub 2016 Aug 2.
  PUBMED    27484481
REFERENCE   2  (bases 1 to 992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkw686"; journalName: "Nucleic Acids Res"; date: 
            "2016-12-15- 15"; volume: "44"; issue: "22"; pages: "10912-10928"
COMMENT     Sequence Label: pRS314_U6_A34G_A35G
FEATURES             Location/Qualifiers
     source          1..992
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(66..346)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     primer_bind     complement(367..386)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     486..508
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(546..564)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gtgctctgca agccgcaaac tttcaccaat ggaccagaac tacctgtgaa attaataaca
       61 gacatactcc aagctgcctt tgtgtgctta atcacgtata ctcacgtgct caatagtcac
      121 caatgccctc cctcttggcc ctctcctttt cttttttcga ccgaattaat tcttaatcgg
      181 caaaaaaaga aaagctccgg atcaagattg tacgtaaggt gacaagctat ttttcaataa
      241 agaatatctt ccactactgc catctggcgt cataactgca aagtacacat atattacgat
      301 gctgttctat taaatgcttc ctatattata tatatagtaa tgtcgtgatc tatggtgcac
      361 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
      421 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
      481 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
      541 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
      601 gacggatcgc ttgcctgtaa cttacacgcg cctcgtatct tttaatgatg gaataatttg
      661 ggaatttact ctgtgtttat ttatttttat gttttgtatt tggattttag aaagtaaata
      721 aagaaggtag aagagttacg gaatgaagaa aaaaaaataa acaaaggttt aaaaaatttc
      781 aacaaaaagc gtactttaca tatatattta ttagacaaga aaagcagatt aaatagatat
      841 acattcgatt aacgataagt aaaatgtaaa atcacaggat tttcgtgtgt ggtcttctac
      901 acagacaaga tgaaacaatt cggcattaat acctgagagc aggaagagca agataaaagg
      961 tagtatttgt tggcgatccc cctagagtct tt
//
