LOCUS       sequence-166108-        1045 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  U6 telestem mutant (A34G/A35G) for in vivo studies.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Rodgers ML, Didychuk AL, Butcher SE, Brow DA, Hoskins AA
  TITLE     A multi-step model for facilitated unwinding of the yeast U4/U6 RNA 
            duplex.
  JOURNAL   Nucleic Acids Res. 2016 Dec 15;44(22):10912-10928. doi: 
            10.1093/nar/gkw686. Epub 2016 Aug 2.
  PUBMED    27484481
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkw686"; journalName: "Nucleic Acids Res"; date: 
            "2016-12-15- 15"; volume: "44"; issue: "22"; pages: "10912-10928"
COMMENT     Sequence Label: pRS314_U6_A34G_A35G
FEATURES             Location/Qualifiers
     source          1..1045
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    95..116
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(131..136,137..154,155..161)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    169..185
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     174..196
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     193..209
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     193..209
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        230..248
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     276..292
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     277..293
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(611..627)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(611..627)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(659..678)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(660..678)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(685..702)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(685..701)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(694..716)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(929..948)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
       61 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
      121 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
      181 aacaatttca cacaggaaac agctatgacc atgattacgc caagctcgaa attaaccctc
      241 actaaaggga acaaaagctg gtaccgggcc ccccctcgag gtcgacggta tcgataagct
      301 tgatatcgaa ttcttcggct actataaata aatgtttttt tcgcaactat gtgttcgcga
      361 agtaaccctt cgtggacatt tggtcggttt gaaacaatac agagatgatc agcagttccc
      421 ctgcataagg atgaaccgtt ttacaaagag atttatttcg ttttttttta tcatcgagtg
      481 aagtatcgtg acttgtacat ttgaagatac ccagcgtaca gcagtgtatc tttatcttcc
      541 tgtatgatat agataactaa catctcgaat agaaaattgt ctcgcgttcg aacctaagcc
      601 tttgctatcg gatccactag ttctagagcg gccgccaccg cggtggagct ccaattcgcc
      661 ctatagtgag tcgtattaca attcactggc cgtcgtttta caacgtcgtg actgggaaaa
      721 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
      781 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
      841 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
      901 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      961 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     1021 agtgctttac ggcacctcga cccca
//
