LOCUS       sequence-16612-e        1294 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 1294)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD90_31
FEATURES             Location/Qualifiers
     source          1..1294
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             108..128
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(218..240)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gggattggnc aaaggaccca aaggtatgtt tcgaatgata ctaacataac atagaacatt
       61 ttcaggagga cccttggagg gtaccgagct cagaaaaaat gactgctcca aagaagaagc
      121 gtaaggtacc ggtgggtgaa gaccagaaac agcacctcga actgagccgc gatattgccc
      181 agcgtttcaa cgcgctgtat ggcgagatcg atcccgtcgt tttacaacgt cgtgactggg
      241 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
      301 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
      361 aatggcgctt tgcctggttt ccggcaccag aagcggtgcc ggaaagctgg ctggagtgcg
      421 atcttcctga ggccgatact gtcgtcgtcc cctcaaactg gcagatgcac ggttacgatg
      481 cgcccatcta caccaacgtg acctatccca ttacggtcaa tccgccgttt gttcccacgg
      541 agaatccgac gggttgttac tcgctcacat ttaatgttga tgaaagctgg ctacaggaag
      601 gccagacgcg aattattttt gatggcgtta actcggcgtt tcatctgtgg tgcaacgggc
      661 gctgggtcgg ttacggccag gtaagtttaa ttaagttgat actaactaac aagatctgat
      721 taattttcag gacagtcgtt tgccgtctga atttgacctg agcgcatttt tacgcgccgg
      781 agaaaaccgc ctcgcggtga tggtgctgcg ctggagtgac ggcagttatc tggaagatca
      841 ggatatgtgg cggatgagcg gcattttccg tgacgtctcg ttgctgcata aaccgactac
      901 acaaatcagc gatttccatg ttgccactcg ctttaatgat gatttcagcc gcgctgtact
      961 ggaggctgaa gttcanatgt gcggcgagtt gcgtgactac ctacgggtaa cagtttcttt
     1021 atggcagggt gaaaacgcag gtcgccagcg gcacngcgcc tttncggngg tgaaattatc
     1081 gatgancgng gnggttatgc cgatcncgtc nactacgtnt gaacgtcaaa aaccnaaant
     1141 gggnaggncn aaatccnaat cnttttcngg gggggtnaan tgcccccccc angccncctt
     1201 tatnaaanaa aanctggnag ncgtttcccn agggggaatn aaangntntt ttnnttaaan
     1261 naancctttt tttnagggnt tancnncnca aatt
//
