LOCUS       sequence-166134-         776 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of ubiquitin-K48R-EGFP fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Tsirigotis M, Zhang M, Chiu RK, Wouters BG, Gray DA
  TITLE     Sensitivity of mammalian cells expressing mutant ubiquitin to 
            protein-damaging agents.
  JOURNAL   J Biol Chem. 2001 Dec 7;276(49):46073-8.
  PUBMED    11598140
REFERENCE   2  (bases 1 to 776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2001-12-7"; volume: "276(49)"; pages: "46073-8"
COMMENT     Sequence Label: pDG279
FEATURES             Location/Qualifiers
     source          1..776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..242)
                     /codon_start=1
                     /product="human ubiquitin variant in which Lys-48 has been 
                     mutated to arginine"
                     /label=ubiquitin-K48R
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGRQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             complement(243..260)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(427..446)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
ORIGIN
        1 cccttgctca ccataccacc tctcagacgc aggaccaggt gcagggtcga ctctttctgg
       61 atgttgtagt cagaaagagt acggccatct tccagctgcc ggcctgcaaa gatgagcctc
      121 tgctggtcgg gggggatgcc ttctttatct tggatcttgg ccttcacatt ttcgatggtg
      181 tcactgggct ccacctccag agtgatggtc ttgccggtca gggtcttcac gaagatctgc
      241 atgtgatggt gatggtggtg gctgcctctc attttaagct tcgtctaaca aaaaagccaa
      301 aaacggccag aatttagcgg acaatttact agtctaacac tgaaaattac atattgaccc
      361 aaatgattac atttcaaaag gtgcctaaaa aacttcacaa aacacactcg ccaaccccga
      421 gcgcatagtt caaaaccgga gcttcagcta cttaagaaga taggtacata aaaccgacca
      481 aagaaactga cgcctcactt atccctcccc tcaccagagg tccggcgcct gtcgattcag
      541 gagagcctac cctaggcccg aaccctgcgt cctgcgacgg agaaaagcct accgcacacc
      601 taccggcagg tggccccacc ctgcattata agccaacaga acgggtgacg tcacgacacg
      661 acgagggcgc gcgctcccaa aggtacgggt gcactgccca acggcaccgc cataactgcc
      721 gcccccgcaa cagacgacaa accgagttct ccagtcagtg acaaacttca cgtcag
//
