LOCUS       sequence-166136-        1008 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Use to synthesize riboprobes for in situ hybridization (ISH) probe 
            for Emx1. Not for gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Parrilla M, Chang I, Degl'Innocenti A, Omura M
  TITLE     Expression of homeobox genes in the mouse olfactory epithelium.
  JOURNAL   J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. 
            Epub 2016 Jun 19.
  PUBMED    27243442
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.24051";
            journalName: "J Comp Neurol"; date: "2016-10-1- 1"; volume: "524"; 
            issue: "14"; pages: "2713-39"
COMMENT     Sequence Label: pISH-Emx1
FEATURES             Location/Qualifiers
     source          1..1008
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      361..370
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      365..374
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(404..423)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(405..423)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(430..447)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(430..446)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(439..461)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      587..967
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(674..693)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     884..905
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 atccaacgcg ttgggagctc tcccatatgg tcgacctgca ggcggccgcg aattcactag
       61 tgattgtgac atcaatgtcc tccccgttgg cctgcttcgt ggcgatgcgc caccggttga
      121 tatggtggga acccttcttc ttctgctcag actccggccc ttcctcttcc agcttctgcc
      181 gtttgtattt tgtcctccga ttctggaacc acaccttcac ctgcgtctcg gagaggctga
      241 ggctgcctgc cagctgcttc cgctcggctc ccaccacgta gtgattcttc tcaaaggctc
      301 gctccagccg cagcagctgc gagggcgaga aggctgtgcg aatccaatcg aattcccgcg
      361 gccgccatgg cggccgggag catgcgacgt cgggcccaat tcgccctata gtgagtcgta
      421 ttacaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
      481 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
      541 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggacgc gccctgtagc
      601 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
      661 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
      721 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
      781 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
      841 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
      901 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
      961 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaac
//
